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Lil3 dimerization and chlorophyll binding in Arabidopsis thaliana
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2015 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 589, no 20, 3064-3070 p.Article in journal (Refereed) Published
Abstract [en]

The two-helix light harvesting like (Lil) protein Lil3 belongs to the family of chlorophyll binding light harvesting proteins of photosynthetic membranes. A function in tetrapyrrol synthesis and stabilization of geranylgeraniol reductase has been shown. Lil proteins contain the chlorophyll a/b-binding motif; however, binding of chlorophyll has not been demonstrated. We find that Lil3.2 from Arabidopsis thaliana forms heterodimers with Lil3.1 and binds chlorophyll. Lil3.2 heterodimerization (25 +/- 7.8 nM) is favored relative to homodimerization (431 +/- 59 nM). Interaction of Lil3.2 with chlorophyll a (231 +/- 49 nM) suggests that heterodimerization precedes binding of chlorophyll in Arabidopsis thatiana. 

Place, publisher, year, edition, pages
2015. Vol. 589, no 20, 3064-3070 p.
Keyword [en]
Light harvesting like protein 3, Chlorophyll a, Microscale thermophoresis, Hydrophobic cluster analysis plot
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Biochemistry and Molecular Biology Biophysics Cell Biology
Identifiers
URN: urn:nbn:se:umu:diva-112268DOI: 10.1016/j.febslet.2015.08.023ISI: 000364604800005PubMedID: 26320415OAI: oai:DiVA.org:umu-112268DiVA: diva2:878052
Available from: 2015-12-08 Created: 2015-12-04 Last updated: 2016-09-14Bibliographically approved

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Shevela, Dmitriy
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Department of Chemistry
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