BabA Protein Expression and Lewis b Binding is Gene Locus-Dependent
2015 (English)In: Helicobacter, ISSN 1083-4389, E-ISSN 1523-5378, Vol. 20, no Suppl. 1, 111-111 p., Abstract no.: P05.06Article in journal, Meeting abstract (Other academic) Published
Background: Helicobacter pylori (Hp) adhesion, BabA, is localized on the bacterial cell surface, binds to the fucosylated Lewis b histo-blood group antigen on gastric epithelial cells and is responsible for bacterial colonization in the gastric milieu. The diverse roles of this bacterial adhesion in Hp-induced pathogenesis remain understudied.
Methods: We have isolated single colonies of Hp from 156 (NUD=97, DU=34, GC=25) patients. babA and babB genes were evaluated by gene/locus-specific PCR. BabA protein expression and Lewis b (Leb) binding capacity were determined by immunoblotting and ELISA, respectively.
Results: Leb binding assay identified 36% of the strains as high binders and the remaining 64% with low binding capacity. All (100%) of the strains in the former group expressed BabA protein at high levels (BabA-H). Of the latter group, 40% were BabA low producers (BabA-L) and the remaining 60% produced no detectable BabA protein (BabA-Neg). The majority (73/88, 83%) of the strains expressing BabA protein were babA gene-positive at locus A versus. those at locus B (15/88, 17%, P = 0.034). The same holds true for Lewis b binding. In other words, the former group constitutes larger numbers (44/50, 88%) of Leb high-binders relative to the latter group (6/50, 12%, P = 0.035). Furthermore, amongst the former group, co-presence of babA and babB genes in locus A reduced the probability of Leb binding (P = 0.0001).
Conclusion: Presence of babA gene in locus A is associated with higher BabA protein expression and Lewis b binding. Therefore, gene/locus-specific PCR seems better suited for the assessment of functional BabA protein.
Place, publisher, year, edition, pages
John Wiley & Sons, 2015. Vol. 20, no Suppl. 1, 111-111 p., Abstract no.: P05.06
Biochemistry and Molecular Biology Biophysics
IdentifiersURN: urn:nbn:se:umu:diva-111506ISI: 000363064400149OAI: oai:DiVA.org:umu-111506DiVA: diva2:881416
European-Helicobacter-Study-Group 28th International Workshop on Helicobacter and Microbiota in Inflammation and Cancer, SEP 24-26, 2015, Nicosia, CYPRUS