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The effect of freeze/thaw cycles on reproducibility of metabolic profiling of marine microalgal extracts using direct infusion high-resolution mass spectrometry (HR-MS)
Universitetet i Tromsø.
Universitetet i Tromsø.
Universitetet i Tromsø.
Universitetet i Tromsø.
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2014 (English)In: Molecules, Vol. 19, no 10, 16373-80 p.Article in journal (Refereed) Published
Abstract [en]

During normal sample preparation, storage in freezers and subsequent freeze/thaw cycles are commonly introduced. The effect of freeze/thaw cycles on the metabolic profiling of microalgal extracts using HR-MS was investigated. Methanolic extracts of monocultures of Arctic marine diatoms were analyzed immediately after extraction, after seven days of storage at -78 degrees C (one freeze/thaw cycle), and after additional seven days at -20 degrees C (two freeze/thaw cycles). Repeated direct infusion high-resolution mass spectrometry analysis of microalgae extracts of the same sample showed that reproducibility was ca. 90% when a fresh (unfrozen) sample was analyzed. The overall reproducibility decreased further by ca. 10% after the first freeze/thaw-cycle, and after one more freeze/thaw cycle the reproducibility decreased further by ca. 7%. The decrease in reproducibility after freeze-thaw cycles could be attributed to sample degradation and not to instrument variability.

Place, publisher, year, edition, pages
2014. Vol. 19, no 10, 16373-80 p.
Keyword [en]
metabolic profiling, metabolomics, microalgae
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Identifiers
URN: urn:nbn:se:umu:diva-112692ISBN: 1420-3049 (Electronic) 1420-3049 (Linking)OAI: oai:DiVA.org:umu-112692DiVA: diva2:882196
Note

Eilertsen, Hans Chr Huseby, Siv Degerlund, Maria Eriksen, Gunilla K Ingebrigtsen, Richard A Hansen, Espen eng Research Support, Non-U.S. Gov't Switzerland 2014/10/15 06:00 Molecules. 2014 Oct 13;19(10):16373-80. doi: 10.3390/molecules191016373.

Available from: 2015-12-14 Created: 2015-12-14 Last updated: 2015-12-14

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http://www.ncbi.nlm.nih.gov/pubmed/25314600

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