The effect of freeze/thaw cycles on reproducibility of metabolic profiling of marine microalgal extracts using direct infusion high-resolution mass spectrometry (HR-MS)
2014 (English)In: Molecules, Vol. 19, no 10, 16373-80 p.Article in journal (Refereed) Published
During normal sample preparation, storage in freezers and subsequent freeze/thaw cycles are commonly introduced. The effect of freeze/thaw cycles on the metabolic profiling of microalgal extracts using HR-MS was investigated. Methanolic extracts of monocultures of Arctic marine diatoms were analyzed immediately after extraction, after seven days of storage at -78 degrees C (one freeze/thaw cycle), and after additional seven days at -20 degrees C (two freeze/thaw cycles). Repeated direct infusion high-resolution mass spectrometry analysis of microalgae extracts of the same sample showed that reproducibility was ca. 90% when a fresh (unfrozen) sample was analyzed. The overall reproducibility decreased further by ca. 10% after the first freeze/thaw-cycle, and after one more freeze/thaw cycle the reproducibility decreased further by ca. 7%. The decrease in reproducibility after freeze-thaw cycles could be attributed to sample degradation and not to instrument variability.
Place, publisher, year, edition, pages
2014. Vol. 19, no 10, 16373-80 p.
metabolic profiling, metabolomics, microalgae
Other Biological Topics
IdentifiersURN: urn:nbn:se:umu:diva-112692ISBN: 1420-3049 (Electronic) 1420-3049 (Linking)OAI: oai:DiVA.org:umu-112692DiVA: diva2:882196
Eilertsen, Hans Chr Huseby, Siv Degerlund, Maria Eriksen, Gunilla K Ingebrigtsen, Richard A Hansen, Espen eng Research Support, Non-U.S. Gov't Switzerland 2014/10/15 06:00 Molecules. 2014 Oct 13;19(10):16373-80. doi: 10.3390/molecules191016373.2015-12-142015-12-142015-12-14