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Oxylipins, endocannabinoids, and related compounds in human milk: levels and effects of storage conditions
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry. Umeå University, Faculty of Medicine, Department of Pharmacology and Clinical Neuroscience.
Umeå University, Faculty of Medicine, Department of Clinical Sciences.
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2016 (English)In: Prostaglandins & other lipid mediators, ISSN 1098-8823, E-ISSN 2212-196X, Vol. 122, 28-36 p.Article in journal (Refereed) Published
Abstract [en]

The presence of fatty acid derived oxylipins, endocannabinoids and related compounds in human milk may be of importance to the infant. Presently, clinically relevant protocols for storing and handling human milk that minimize error and variability in oxylipin and endocannabinoid concentrations are lacking. In this study, we compared the individual and combined effects of the following storage conditions on the stability of these fatty acid metabolites in human milk: state (fresh or frozen), storage temperature (4 °C, -20 °C or -80 °C), and duration (1 day, 1 week or 3 months). Thirteen endocannabinoids and related compounds, as well as 37 oxylipins were analyzed simultaneously by liquid chromatography coupled to tandem mass spectrometry. Twelve endocannabinoids and related compounds (2–111 nM) and 31 oxylipins (1.2 pM–1242 nM) were detected, with highest levels being found for 2-arachidonoylglycerol and 17(R)-hydroxydocosahexaenoic acid, respectively. The concentrations of most endocannabinoid-related compounds and oxylipins were dependent on storage condition, and especially storage at 4 °C introduced significant variability. Our findings suggest that human milk samples should be analyzed immediately after, or within one day of collection (if stored at 4 °C). Storage at -80 °C is required for long-term preservation, and storage at -20 °C is acceptable for no more than one week. These findings provide a protocol for investigating the oxylipin and endocannabinoid metabolome in human milk, useful for future milk-related clinical studies.

Place, publisher, year, edition, pages
Elsevier, 2016. Vol. 122, 28-36 p.
Keyword [en]
quantitative, LC-MS/MS, eicosanoids, endocannabinoids, human milk
National Category
Biochemistry and Molecular Biology Cell Biology Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:umu:diva-112814DOI: 10.1016/j.prostaglandins.2015.11.002ISI: 000372762300004PubMedID: 26656029OAI: oai:DiVA.org:umu-112814DiVA: diva2:882669
Available from: 2015-12-15 Created: 2015-12-15 Last updated: 2016-04-25Bibliographically approved

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Wu, JungfangGouveia-Figueira, SandraDomellöf, MagnusNording, Malin
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Department of ChemistryDepartment of Pharmacology and Clinical NeuroscienceDepartment of Clinical Sciences
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Prostaglandins & other lipid mediators
Biochemistry and Molecular BiologyCell BiologyMedical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

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