umu.sePublications
Change search
ReferencesLink to record
Permanent link

Direct link
A Multivariate Approach Using Attenuated Total Reflectance Mid-infrared Spectroscopy To Measure the Surface Mannoproteins and beta-Glucans of Yeast Cell Walls during Wine Fermentations
Show others and affiliations
2015 (English)In: Journal of Agricultural and Food Chemistry, ISSN 0021-8561, E-ISSN 1520-5118, Vol. 63, no 45, 10054-10063 p.Article in journal (Refereed) PublishedText
Abstract [en]

Yeast cells possess a cell wall comprising primarily glycoproteins, mannans, and glucan polymers. Several yeast phenotypes relevant for fermentation, wine processing, and wine quality are correlated with cell wall properties. To investigate the effect of wine fermentation on cell wall composition, a study was performed using mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA). A total of 40 yeast strains were evaluated, including Saccharomyces strains (laboratory and industrial) and non-Saccharomyces species. Cells were fermented in both synthetic MS300 and Chardonnay grape must to stationery phase, processed, and scanned in the MIR spectrum. PCA of the fingerprint spectral region showed distinct separation of Saccharomyces strains from non-Saccharomyces species; furthermore, industrial wine yeast strains separated from laboratory strains. PCA loading plots and the use of OPLS-DA to the data sets suggested that industrial strains were enriched with cell wall proteins (e.g., mannoproteins), whereas laboratory strains were composed mainly of mannan and glucan polymers.

Place, publisher, year, edition, pages
2015. Vol. 63, no 45, 10054-10063 p.
Keyword [en]
yeast cell wall, wine fermentation, mannoproteins, glucans, spectroscopy, multivariate analysis
National Category
Food Science
Identifiers
URN: urn:nbn:se:umu:diva-113438DOI: 10.1021/acs.jafc.5b03154ISI: 000365149100020PubMedID: 26488434OAI: oai:DiVA.org:umu-113438DiVA: diva2:885388
Available from: 2015-12-18 Created: 2015-12-18 Last updated: 2015-12-18Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Trygg, Johan
By organisation
Department of Chemistry
In the same journal
Journal of Agricultural and Food Chemistry
Food Science

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 33 hits
ReferencesLink to record
Permanent link

Direct link