Enterococcus faecalis Susceptibility to Alkaline Environments – a pilot study
Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Enterococcus faecalis has been shown to manifest the oral cavity and may be the cause of persistent infections in already root canal treated teeth. It has the capability of surviving in a high alkaline environment and undergoes starvation in a minimal metabolic state. The aim of the present study is to evaluate the antibacterial effect of pH 7.1, 10.0, 10.7 and 11.2 on Enterococcus faecalis during 7 days (168 hours). The hypothesis is that the stress induced by an alkaline environment will negatively affect the growth and survival of Enterococcus faecalis. The key methods used in this pilot study are cultivation on agar plates and optical density for measuring the bacterial growth and pH to control the experimental environment. The results show that pH 10.0 retards growth of Enterococcus faecalis, whereas no growth could be seen at pH 10.7 and pH 11.2, however pH 7.1 indicated initial growth. The initial growth stopped after 8 h and the reason for that might be due to lack of nutrition. The conclusion of this study is that a high alkaline intra canal pH may kill or retard the growth of Enterococcus faecalis.
Place, publisher, year, edition, pages
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:umu:diva-113599OAI: oai:DiVA.org:umu-113599DiVA: diva2:886027
Ebrahimi, MajidBrundin, Malin