Change search
ReferencesLink to record
Permanent link

Direct link
Sediment extraction and clean-up for organic phosphorus analysis by electrospray ionization tandem mass spectrometry
Show others and affiliations
2008 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 74, no 5, 1175-1183 p.Article in journal (Refereed) PublishedText
Abstract [en]

A method to prepare NaOH sediment extracts for organic P compound analysis with electrospray ionization tandem mass spectrometry (ESI-MS-MS) was developed on natural samples. Ion exchange, rotary evaporation and mass cut-off filtering proved to be suitable for sample preparation. Samples were analyzed with ESI-MS-MS, and reproducibility and repeatability of the method was calculated. In addition, P-31-nuclear magnetic resonance Spectroscopy (P-31 NMR)was used to measure recovery of different P compound groups such as orthophosphate (Ortho-P), orthophosphate monoesters (Monoester-P), orthophosphate diesters (Diester-P) and pyrophosphates (Pyro-P). The developed sample preparation method resulted in an easy-to-spray liquid for the ESI-MS-MS instrumentation. The overall P recovery was 65% and P-31 NMR showed that Diester-P, possibly in the form of DNA, was apparently lost through the filtering step most likely due to their size. Variances in the total intensities of the MS scans (relative standard deviation (R.S.D.) 35-54%) were for about 50% due to repeated MS runs. Covariances of the peaks in the MS spectra were calculated to be for about 30% due to the sample preparation procedure. Finally, with the ESI-MS-MS approach, 11 peaks in the mass spectra were found likely to represent phosphate containing compounds. (c) 2007 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
2008. Vol. 74, no 5, 1175-1183 p.
Keyword [en]
organic phosphorus, sediment, sample preparation, ESI-MS-MS, P-31 NMR
National Category
Analytical Chemistry
URN: urn:nbn:se:umu:diva-113613DOI: 10.1016/j.talanta.2007.08.030ISI: 000253514700013PubMedID: 18371767OAI: diva2:886068
Available from: 2015-12-21 Created: 2015-12-21 Last updated: 2016-02-15

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
De Brabandere, Heidi
In the same journal
Talanta: The International Journal of Pure and Applied Analytical Chemistry
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 43 hits
ReferencesLink to record
Permanent link

Direct link