Today people spend a lot of time in sedentary behavior, leading to a higher risk for different negative health outcomes. It is common that dentists sit for a long time during their working day. The purpose of this study was to compare and describe the percentage of time working dentists and dental students spend in a sitting position and how many times they change from a seated position to a standing position during a work day. The hypothesis was that dentists spend less time in a sitting position and alternate more between sitting and standing than dental students. Eight dentists and nine dental students were recorded two days each using a triaxial accelerometer attached to the thigh. With the accelerometer it is possible to discriminate between sitting and standing by calculating angle the accelerometer adopts in relation to gravity. Results showed that dentists were seated more than dental students. No difference was found when comparing how many times the groups changed from a seated position to a standing, and the hypothesis was therefore rejected. Recommendations for dentists and dental students are to spend less time in a seated position during work days. It is suggested that future research should focus on the correlation between sitting time and health risks, like how musculoskeletal disorders and cardiovascular disease correlates with amount of time spent in a sitting position for dentists.