Development of a Study design: Evaluation of Guava (Psidium guajava) Compounds as Inhibitor for Aggregatibacter actinomycetemcomitans Leukotoxin induced Inflammation
Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Highly leukotoxic Aggregatibacter actinomycetemcomitans associated with aggressive periodontitis occur in high frequency in Ghana. Carriers of this bacterium have an increased risk of developing rapid attachment loss. The possibility for dental care in developing countries is incomplete why simple preventive strategies for self-care are of great importance. Studies have shown that components from guava (Psidium guajava) efficiently neutralize A. actinomycetemcomitans leukotoxin. The aim of this work was to develop a study design where components from guava are tested on adolescents in Ghana to prevent attachment loss initiated by A. actinomycetemcomitans. Further, to evaluate if biomarkers in saliva can reflect lekotoxin neutralization.
Data from earlier studies in Ghana were used for statistical calculations of the study design. Saliva samples from adolescents in Ghana were analyzed for presence of A. actinomycetemcomitans by quantitative PCR, and for the periodontitis associated biomarkers matrix metalloproteinase-8 (MMP-8) and Interleukin-1 beta (IL-1β) by ELISA. Both MMP-8 and IL-1β was possible to detect in the saliva samples.
The previous work showed that the study design should involve 205 participants from public school in two groups, one treatment group (guava) and one control group. After 2 years there will be a follow up with a clinical screening on first molars. The results from the follow up can be compared with results from earlier studies on the same population.
If the results from the follow up study show that guava is efficient in vivo, chewing sticks from guava trees might be a future preventive tool for leukotoxin neutralization.
Place, publisher, year, edition, pages
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:umu:diva-113644OAI: oai:DiVA.org:umu-113644DiVA: diva2:886278