Eotaxin and MCP-1 in Periodontitis
Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Periodontitis is a chronic inflammatory disease of tooth supporting tissues resulting in periodontal tissue destruction, which may ultimately lead to tooth loss. The pathogenetic mechanisms of periodontitis are not fully elucidated. The disease is characterized by continuous leukocyte infiltration, likely mediated by local cytokine and chemokine production. There are no serologic biomarkers for the diagnosis of periodontitis, which is based solely on the degree of local tissue destruction. The aim of this study was to identify serological biomarkers for periodontitis by analyzing a spectrum of cytokines in serum from subjects with and without periodontitis and also to investigate if the detected cytokines were expressed by gingival fibroblasts, the most abundant cell type in the inflamed periodontium. We mapped serum cytokine and chemokine levels from periodontitis subjects and healthy controls. We used multivariate partial least squares (PLS) modeling and identified monocyte chemoattractant protein-1 (MCP-1) and eotaxin as robustly associated with periodontitis along with C-reactive protein (CRP), years of smoking and age, whereas the number of remaining teeth and a high education level was associated with being healthy. We detected higher MCP-1 protein levels in inflamed gingival connective tissue compared to healthy but the eotaxin levels were undetectable. Primary human gingival fibroblasts displayed strongly increased expression of MCP-1 and eotaxin mRNA and protein when challenged with tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), key mediators of periodontal inflammation. Furthermore, targeting their expression in gingival fibroblasts could result in reduced leukocyte infiltration and inflammation in periodontitis, and maybe prevent tooth loss.
Place, publisher, year, edition, pages
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:umu:diva-113659OAI: oai:DiVA.org:umu-113659DiVA: diva2:887128