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The Efficacy of Nd:YAG Laser Compared to Sodium Hypochlorite in Reducing the Amount of Enterococcus faecalis in Ex Vivo Root Canals
Umeå University, Faculty of Medicine, Department of Odontology.
Umeå University, Faculty of Medicine, Department of Odontology.
2015 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

A common problem in endodontic treatment is to totally eliminate bacteria, especially when it comes to retreatment of teeth with persistent infection and periapical lesions. In these cases Enterococcus faecalis is the most prevalent bacteria, often found in dentinal tubules. Laser is a relatively new technique used in endodontic treatment, a technique that is presumed to eliminate bacteria in dentinal tubules and effectively remove smear layer in the canal.


The aim of this study was to evaluate the efficacy of Nd:YAG laser in reducing the presence of E. faecalis compared to conventional methods with mechanical instrumentation in combination with chemical irrigants and insertions. Twelve extracted sterilized teeth were inoculated with a suspension of E. faecalis for a period of two weeks. The teeth were randomly allocated to three test groups: Conventional treatment with sodium hypochlorite (CT, n = 5), laser treatment and saline solution (LT, n = 5) and positive control with saline solution (PC, n = 2). Bacterial samples were collected before and after treatment and analyzed using culture technique.


There was no significant difference in bacterial reduction between the three test groups CT, LT and PC. More studies are required to evaluate the efficacy Nd:YAG laser in bacterial reduction.

Place, publisher, year, edition, pages
National Category
Medical and Health Sciences
URN: urn:nbn:se:umu:diva-113662OAI: diva2:887335
Educational program
Dentistry Programme
Available from: 2015-12-22 Created: 2015-12-22 Last updated: 2016-02-08Bibliographically approved

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