Independent thesis Advanced level (professional degree), 20 credits / 30 HE credits
In the oral cavity, inflammatory processes are caused by bacterial adhesion to non-shedding surfaces. If these surfaces could be modified to prohibit bacterial adhesion, inflammation could be prevented. In this study, bacterial adhesion to a superhydrophobic alkyl ketene dimer (AKD) surface was tested. The AKD-surface, produced by spraying an aerosol onto an existing surface (e.g. a dental prosthesis), has been shown to have largely enhanced hydrophobic characteristics, thus potentially repellant of bacteria.
In this study, bacterial adhesion onto the AKD-surface produced on glass was compared to bacterial adhesion onto surfaces of glass, polyethylenterephthalat-plastic, titanium and biological surfaces, i.e. enamel and dentin. Streptococcus mutans and Streptococcus sanguinis were labeled with fluorescent antibodies, fluorescein isothiocyanate (FITC), and applied to the different surfaces in four tests; control and three different tests where the surfaces were pretreated with blocking buffer, non-secretor saliva and secretor saliva. The areas where bacteria had adhered were quantified and compared. The tooth surface had to be excluded from the study because of optical interference with the FITC labeled bacteria. The AKD-surface was damaged during the experiments and could only be included in the control.
Overall, S. sanguinis adhered to a larger extent compared to S. mutans, with some exceptions. More bacteria adhered after pretreatment with non-secretor saliva compared to secretor saliva. It was concluded that the AKD-surface was unsuitable for use in the oral cavity. Further studies on anti-adhesive coatings that can prevent inflammation in the oral cavity are welcome.