Change search
ReferencesLink to record
Permanent link

Direct link
The Arabidopsis DELAY OF GERMINATION 1 gene affects ABSCISIC ACID INSENSITIVE 5 (ABI5) expression and genetically interacts with ABI3 during Arabidopsis seed development
Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC). Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Department of Molecular Plant Physiology, Utrecht University, CH, Utrecht, The Netherlands.ORCID iD: 0000-0002-5605-7984
Show others and affiliations
2016 (English)In: The Plant Journal, ISSN 0960-7412, E-ISSN 1365-313X, Vol. 85, no 4, 451-465 p.Article in journal (Refereed) Published
Abstract [en]

The seed expressed gene DELAY OF GERMINATION (DOG) 1 is absolutely required for the induction of dormancy. Next to a non-dormant phenotype, the dog1-1 mutant is also characterized by a reduced seed longevity suggesting that DOG1 may affect additional seed processes as well. This aspect however, has been hardly studied and is poorly understood. To uncover additional roles of DOG1 in seeds we performed a detailed analysis of the dog1 mutant using both transcriptomics and metabolomics to investigate the molecular consequences of a dysfunctional DOG1 gene. Further, we used a genetic approach taking advantage of the weak aba insensitive (abi) 3-1 allele as a sensitized genetic background in a cross with dog1-1. DOG1 affects the expression of hundreds of genes including LATE EMBRYOGENESIS ABUNDANT and HEAT SHOCK PROTEIN genes which are affected by DOG1 partly via control of ABI5 expression. Furthermore, the content of a subset of primary metabolites, which normally accumulate during seed maturation, was found to be affected in the dog1-1 mutant. Surprisingly, the abi3-1 dog1-1 double mutant produced green seeds which are highly ABA insensitive, phenocopying severe abi3 mutants, indicating that dog1-1 acts as an enhancer of the weak abi3-1 allele and thus revealing a genetic interaction between both genes. Analysis of the dog1 and dog1 abi3 mutants revealed additional seed phenotypes and therefore we hypothesize that DOG1 function is not limited to dormancy but that it is required for multiple aspects of seed maturation, in part by interfering with ABA signalling components.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2016. Vol. 85, no 4, 451-465 p.
Keyword [en]
DELAY OF GERMINATION, dormancy, seed development, seed maturation, ABA insensitive, abscisic acid, Arabidopsis thaliana (Arabidopsis)
National Category
URN: urn:nbn:se:umu:diva-114427DOI: 10.1111/tpj.13118ISI: 000370339000001PubMedID: 26729600OAI: diva2:895334
Available from: 2016-01-18 Created: 2016-01-18 Last updated: 2016-03-17Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Hanson, Johannes
By organisation
Umeå Plant Science Centre (UPSC)Department of Plant Physiology
In the same journal
The Plant Journal

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 308 hits
ReferencesLink to record
Permanent link

Direct link