Prediction of the next highly pathogenic avian influenza pandemic that can cause illness in humans
2015 (English)In: Infectious diseases of poverty, ISSN 2049-9957, Vol. 4, 50Article in journal (Refereed) PublishedText
Background: In recent years, avian influenza viruses (AIVs) have seriously threatened human health. Questions such as: why do AIVs infect humans?, how quickly can an AIV become pandemic?, and which virus is the most dangerous? cannot be sufficiently answered using current bioinformatic studies. Method: Secondary structures and energies of representative 5'-untranslated region (UTR) of the HA gene were calculated. Then their secondary structures and energies were re-calculated after one or two nucleotide substitutions were introduced into the HA 5'-UTR. Phylogenetic trees on the basis of hemagglutinin (HA) and polymerase basic protein 2 (PB2) amino acid sequences and HA 5'-UTR nucleotide sequences were constructed. The connection between the energy and translation efficiency of 5'-UTR was confirmed by in vitro coupled transcription/translation assay. Results: The simplicity of the secondary structure of the 5'-UTR of the HA gene determines the overall virus replication rate and transmission potential. Point mutation assays show that the 5'-UTR sequences of the HA gene in the influenza subtypes H2N2, H3N2, and H7N9 have greater variation potentials than other virus subtypes. Conclusion: Some high-virulent strains of avian influenza might emerge in the next two to three years. The H2N2 subtype, once disappeared in humans, may stage a comeback. The current outbreak of H7N9 may become pandemic and cause even more deaths, if one or two bases are substituted in the 5'-UTR sequence of the HA gene.
Place, publisher, year, edition, pages
BioMed Central, 2015. Vol. 4, 50
Avian influenza viruses, Hemagglutinin, Point mutation, Translation efficiency, 5'-untranslated region
IdentifiersURN: urn:nbn:se:umu:diva-114640DOI: 10.1186/s40249-015-0083-8ISI: 000367174500001PubMedID: 26612517OAI: oai:DiVA.org:umu-114640DiVA: diva2:899795