The Effects of 1,25-Dihydroxyvitamin D3 on the Expression of COX- Enzymes in Human Gingival Fibroblast
Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
1, 25-Dihydroxyvitamin D3 has been shown to have regulatory effects on bone re-modeling and on the immune system including anti-inflammatory effects. Prostaglandins are well known for having pro-inflammatory effects and for being involved in bone resorption in periodontitis. Gingival fibroblasts express COX-enzymes which are key enzymes in prostaglandin biosynthesis. The hypothesis in the present study was that 1, 25-Dihydroxyvitamin D3 has an effect on the production of COX-enzymes in gingival fibroblasts which may explain the anti-inflammatory effects of this hormone. Human gingival fibroblasts were obtained from clinically healthy gingiva and used in tests designed to see if the COX-2-enzyme was affected when human gingival fibroblasts were exposed to Vitamin D3.
1, 25-dihydroxyvitamin D3 (10-8M) time-dependently inhibited Cox-2 mRNA expression induced by TNF- α (50 ng/ml). The human gingival fibroblasts expressed both vitamin D receptor and its dimerizing partner retinoic X receptor-α.
The data points toward that circulatory 1, 25-dihydroxyvitamin D3, the most active metabolite of vitamin D, may decrease prostaglandin production through decreased expression of COX-2-enzymes in human gingival fibroblast which may be a mechanism by which vitamin D acts anti-inflammatory.
Place, publisher, year, edition, pages
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:umu:diva-116066OAI: oai:DiVA.org:umu-116066DiVA: diva2:901427