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Design and Calibration of Optical Tweezers Using the Equipartition Theorem
Umeå University, Faculty of Science and Technology, Department of Physics.
2015 (English)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

The aim was to construct an optical tweezers system for moving small polystyrene particles and apply the equipartition theorem for the calibration of trap stiffness. The construction of the optical tweezers system was based on simulations using a ray-tracing program to achieve desired expansion of the beam and limited amounts of aberrations. A position sensitive detector (PSD) is used to record the positions of the bead trapped in the optical tweezers. The calibration between the position of the particle and the relative position on the PSD was based on the power spectrum method. We got the stiffness of the optical tweezers 0.28 pN/μm and 0.29 pN/μm in the x and the y directions with 1 μm beam using a 10 mW He-Ne laser.

Place, publisher, year, edition, pages
National Category
Atom and Molecular Physics and Optics
URN: urn:nbn:se:umu:diva-117077OAI: diva2:904774
Subject / course
Fysik C - Examensarbete
Available from: 2016-02-19 Created: 2016-02-19 Last updated: 2016-02-19Bibliographically approved

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