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Identification of molecular signatures specific for distinct cranial sensory ganglia in the developing chick
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM). Department of Zoology, University of Oxford, Oxford, UK.
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2016 (English)In: Neural Development, ISSN 1749-8104, Vol. 11, 3Article in journal (Refereed) PublishedText
Abstract [en]

Background: The cranial sensory ganglia represent populations of neurons with distinct functions, or sensory modalities. The production of individual ganglia from distinct neurogenic placodes with different developmental pathways provides a powerful model to investigate the acquisition of specific sensory modalities. To date there is a limited range of gene markers available to examine the molecular pathways underlying this process.

Results: Transcriptional profiles were generated for populations of differentiated neurons purified from distinct cranial sensory ganglia using microdissection in embryonic chicken followed by FAC-sorting and RNAseq. Whole transcriptome analysis confirmed the division into somato- versus viscerosensory neurons, with additional evidence for subdivision of the somatic class into general and special somatosensory neurons. Cross-comparison of distinct ganglia transcriptomes identified a total of 134 markers, 113 of which are novel, which can be used to distinguish trigeminal, vestibulo-acoustic and epibranchial neuronal populations. In situ hybridisation analysis provided validation for 20/26 tested markers, and showed related expression in the target region of the hindbrain in many cases.

Conclusions: One hundred thirty-four high-confidence markers have been identified for placode-derived cranial sensory ganglia which can now be used to address the acquisition of specific cranial sensory modalities.

Place, publisher, year, edition, pages
2016. Vol. 11, 3
Keyword [en]
Cranial sensory ganglia, Viscerosensory neuron, Somatosensory neuron, Cell type markers, Chicken
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
URN: urn:nbn:se:umu:diva-116738DOI: 10.1186/s13064-016-0057-yISI: 000368768000001OAI: diva2:904832
Available from: 2016-02-19 Created: 2016-02-11 Last updated: 2016-02-24Bibliographically approved

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Patthey, Cedric
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Umeå Centre for Molecular Medicine (UCMM)
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