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Identification of molecular signatures specific for distinct cranial sensory ganglia in the developing chick
Umeå universitet, Medicinska fakulteten, Umeå centrum för molekylär medicin (UCMM). Department of Zoology, University of Oxford, Oxford, UK.
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2016 (Engelska)Ingår i: Neural Development, ISSN 1749-8104, Vol. 11, artikel-id 3Artikel i tidskrift (Refereegranskat) Published
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Abstract [en]

Background: The cranial sensory ganglia represent populations of neurons with distinct functions, or sensory modalities. The production of individual ganglia from distinct neurogenic placodes with different developmental pathways provides a powerful model to investigate the acquisition of specific sensory modalities. To date there is a limited range of gene markers available to examine the molecular pathways underlying this process.

Results: Transcriptional profiles were generated for populations of differentiated neurons purified from distinct cranial sensory ganglia using microdissection in embryonic chicken followed by FAC-sorting and RNAseq. Whole transcriptome analysis confirmed the division into somato- versus viscerosensory neurons, with additional evidence for subdivision of the somatic class into general and special somatosensory neurons. Cross-comparison of distinct ganglia transcriptomes identified a total of 134 markers, 113 of which are novel, which can be used to distinguish trigeminal, vestibulo-acoustic and epibranchial neuronal populations. In situ hybridisation analysis provided validation for 20/26 tested markers, and showed related expression in the target region of the hindbrain in many cases.

Conclusions: One hundred thirty-four high-confidence markers have been identified for placode-derived cranial sensory ganglia which can now be used to address the acquisition of specific cranial sensory modalities.

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2016. Vol. 11, artikel-id 3
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Cranial sensory ganglia, Viscerosensory neuron, Somatosensory neuron, Cell type markers, Chicken
Nationell ämneskategori
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
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URN: urn:nbn:se:umu:diva-116738DOI: 10.1186/s13064-016-0057-yISI: 000368768000001OAI: oai:DiVA.org:umu-116738DiVA, id: diva2:904832
Tillgänglig från: 2016-02-19 Skapad: 2016-02-11 Senast uppdaterad: 2018-06-07Bibliografiskt granskad

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Patthey, Cedric

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Neural Development
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

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