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Role of melanin pigment in expression of Vibrio cholerae virulence factors
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).ORCID iD: 0000-0002-4754-4676
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
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2009 (English)In: Infection and Immunity, ISSN 0019-9567, E-ISSN 1098-5522, Vol. 77, no 3, 935-942 p.Article in journal (Refereed) PublishedText
Abstract [en]

We identified the mutated gene locus in a pigment-overproducing Vibrio cholerae mutant of strain A1552. The deduced gene product is suggested to be an oxidoreductase based on partial homology to putative homogentisate 1,2-dioxygenase in Pseudomonas aeruginosa and Mesorhizobium loti, and we propose that the gene VC1345 in the V. cholerae genome be denoted hmgA in accordance with the nomenclature for other species. The hmgA:: mini-Tn5 mutant showed a nonpigmented phenotype after complementation with a plasmid clone carrying the WT hmgA(+) locus. Microarray transcription analysis revealed that expression of hmgA and the neighboring genes encoding a postulated two-component sensor system was growth phase dependent. Results from quantitative reverse transcription-PCR analysis showed that hmgA operon expression was reduced in the rpoS mutant, but pigment production by the WT V. cholerae or the hmgA mutant was not detectably influenced by the stationary-phase regulator RpoS. The pigmented mutant showed increased UV resistance in comparison with the WT strain. Interestingly, the pigment-producing mutant expressed more toxin-coregulated pilus and cholera toxin than WT V. cholerae. Moreover, the hmgA mutant showed a fivefold increase in the ability to colonize the intestines of infant mice. A possible mechanism by which pigment production might cause induction of the ToxR regulon due to generation of hydrogen peroxide was supported by results from tests showing that externally supplied H2O2 led to higher TcpA levels. Taken together, our findings suggest that melanin pigment formation may play a role in V. cholerae virulence factor expression.

Place, publisher, year, edition, pages
American Society for Microbiology , 2009. Vol. 77, no 3, 935-942 p.
Keyword [en]
mannose-sensitive hemagglutinin, homogentisic acid, Escherichia coli, EL-TOR, Legionella-pneumophila, toxin, colonization, gene, pathogenesis, arthritis
National Category
Infectious Medicine Immunology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-116019DOI: 10.1128/IAI.00929-08ISI: 000263416700002PubMedID: 19103773OAI: oai:DiVA.org:umu-116019DiVA: diva2:905394
Available from: 2016-02-22 Created: 2016-02-08 Last updated: 2016-02-22Bibliographically approved

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Valeru, Soni PriyaRompikuntal, Pramod KumarIshikawa, TakahikoVaitkevicius, KarolisWai, Sun Nyunt
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Department of Molecular Biology (Faculty of Medicine)
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Infection and Immunity
Infectious MedicineImmunology in the medical area

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