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Exposure of HepG2 cells to low levels of PAH-containing extracts from contaminated soils results in unpredictable genotoxic stress responses
Umeå University, Faculty of Science and Technology, Department of Chemistry.
2009 (English)In: Environmental and Molecular Mutagenesis, ISSN 0893-6692, E-ISSN 1098-2280, Vol. 50, no 4, 337-348 p.Article in journal (Refereed) PublishedText
Abstract [en]

Contaminated soil is a serious environmental problem, constituting a risk to humans and the environment. Polycyclic aromatic hydrocarbons (PAHs) are often present at contaminated sites. However, risk levels are difficult to estimate because of the complexity of contaminants present. Here, we compare cellular effects of extracts from contaminated soils collected at six industrial settings in Sweden. Chemical analysis showed that all soils contained complex mixtures of PAHs and oxy-PAHs. Western blotting and immunocytochemistry were used to investigate DNA damage signaling in HepG2 cells exposed to extracts from these soils. The effects on phosphorylated Mdm2, p53, Erk, H2AX, 53BP1, and Chk2, cell cycle regulating proteins (cyclin D1 and p21), and cell proliferation were compared. We found that most soil extracts induced phosphorylation of Mdm2 at the 2A10 epitope at low concentrations. This is in line with previous studies suggesting that this endpoint reflects readily repaired DNA-damage. However, we found concentration and time-dependent gamma H2AX and 53BP1 responses that were sustained for 48 hr. These endpoints may reflect the presence of different types of persistent DNA-damage. High concentrations of soil extracts decreased cyclin D1 and increased p21 response, indicating cell cycle arrest. Phosphorylation of Mdm2 at Ser 166, which attenuates the p53 response and is induced by many tumor promoters, was induced in a time-dependent manner and was associated with Erk phosphorylation. Taken together, the PAH extracts elicited unpredictable signaling responses that differed between samples. More polar compounds, i.e., oxy-PAHs, also contributed to the complexity.

Place, publisher, year, edition, pages
John Wiley & Sons, 2009. Vol. 50, no 4, 337-348 p.
Keyword [en]
oxy-PAH, Mdm2, H2AX, 53BP1
National Category
Environmental Sciences Genetics
Identifiers
URN: urn:nbn:se:umu:diva-116006DOI: 10.1002/em.20486ISI: 000265172200007PubMedID: 19306413OAI: oai:DiVA.org:umu-116006DiVA: diva2:905754
Available from: 2016-02-23 Created: 2016-02-08 Last updated: 2016-02-23Bibliographically approved

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Lundstedt, Staffan
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