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Quantification for total demethylation potential of environmental samples utilizing the EGFP reporter gene
Umeå University, Faculty of Science and Technology, Department of Chemistry.
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2016 (English)In: Journal of Hazardous Materials, ISSN 0304-3894, E-ISSN 1873-3336, Vol. 306, 278-285 p.Article in journal (Refereed) PublishedText
Abstract [en]

Abstract The demethylation potential of pollutants is arguably an innate component of their toxicity in environmental samples. A method was developed for determining the total demethylation potential of food samples (TDQ). The demethylation epigenetic toxicity was determined using the Hep G2 cell line transfected with pEGFP-C3 plasmids containing a methylated promoter of the EGFP reporter gene. The total demethylation potential of the sample extracts (the 5-AZA-CdR demethylation toxic equivalency) can be quantified within one week by using a standard curve of the 5-AZA-CdR demethylation agent. To explore the applicability of TDQ for environmental samples, 17 groundwater samples were collected from heavy polluted Kuihe river and the total demethylation potentials of the sample extracts were measured successfully. Meaningful demethylation toxic equivalencies ranging from 0.00050 to 0.01747 μM were found in all groundwater sample extracts. Among 19 kinds of inorganic substance, As and Cd played important roles for individual contribution to the total demethylation epigenetic toxicity. The TDQ assay is reliable and fast for quantifying the DNA demethylation potential of environmental sample extracts, which may improve epigenetic toxicity evaluations for human risk assessment, and the consistent consuming of groundwater alongside the Kuihe river pose unexpected epigenetic health risk to the local residents.

Place, publisher, year, edition, pages
Elsevier, 2016. Vol. 306, 278-285 p.
Keyword [en]
Demethylation potential, Environmental sample, Kuihe river, Epigenetic toxicity, EGFP
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:umu:diva-117443DOI: 10.1016/j.jhazmat.2015.12.033ISI: 000374803400030OAI: oai:DiVA.org:umu-117443DiVA: diva2:907711
Available from: 2016-02-29 Created: 2016-02-29 Last updated: 2016-06-27Bibliographically approved

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Tysklind, Mats
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