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Deletion of the gene family of small chlorophyll-binding proteins (ScpABCDE) offsets C/N homeostasis in Synechocystis PCC 6803
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
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2016 (English)In: Biochimica et Biophysica Acta - Bioenergetics, ISSN 0005-2728, E-ISSN 1879-2650, Vol. 1857, no 4, 396-407 p.Article in journal (Refereed) PublishedText
Abstract [en]

In the family of chlorophyll binding proteins, single helix small CAB-like proteins (SCPs) are found in all organisms performing oxygenic photosynthesis. Here, we investigated the function of these stress-inducible proteins in the cyanobacterium Synechocystis sp. PCC 6803. We compared physiological, proteome and transcriptome traits of a Photosystem I (PSI) deletion strain, which constitutively induces SCPs, and a PSI-less/ScpABCDE− without SCPs. The SCP mutant cells were larger in size, showed irregular thylakoid structure and differed in cell-surface morphology. Deletion of scp genes strongly affected the carbon (C) and nitrogen (N) balance, resulting in accumulation of carbohydrates and a decrease in N-rich compounds (proteins and chlorophyll). Data from transcriptomic and metabolomic experiments revealed a role of SCPs in the control of chlorophyll biosynthesis. Additionally, SCPs diminished formation of reactive oxygen species, thereby preventing damage within Photosystem II. We conclude that the lack of SCP-function to remove free chlorophyll under stress conditions has a large impact on the metabolism of the entire cell.

Place, publisher, year, edition, pages
Elsevier, 2016. Vol. 1857, no 4, 396-407 p.
Keyword [en]
Small chlorophyll-proteins, High light protection, Pleiotropic effects, C/N metabolism, Cyanobacterium (Synechocystis sp. strain PCC 6803)
National Category
Chemical Sciences Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-117444DOI: 10.1016/j.bbabio.2015.11.011ISI: 000372675600009OAI: oai:DiVA.org:umu-117444DiVA: diva2:907717
Available from: 2016-02-29 Created: 2016-02-29 Last updated: 2016-06-03Bibliographically approved

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Tibiletti, TaniaHernández-Prieto, Miguel A.Funk, Christiane
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