Tissue sample stability: thawing effect on multi-organ samples
2016 (English)In: Metabolomics, ISSN 1573-3882, E-ISSN 1573-3890, Vol. 12, no 2, 19Article in journal (Refereed) Published
Correct handling of samples is essential in metabolomic studies. Improper handling and prolonged storage of samples has unwanted effects on the metabolite levels. The aim of this study was to identify the effects that thawing has on different organ samples. Organ samples from gut, kidney, liver, muscle and pancreas were analyzed for a number of endogenous metabolites in an untargeted metabolomics approach, using gas chromatography time of flight mass spectrometry at the Swedish Metabolomics Centre, Umeå University, Sweden. Multivariate data analysis was performed by means of principal component analysis and orthogonal projection to latent structures discriminant analysis. The results showed that the metabolic changes caused by thawing were almost identical for all organs. As expected, there was a marked increase in overall metabolite levels after thawing, caused by increased protein and cell degradation. Cholesterol was one of the eight metabolites found to be decreased in the thawed samples in all organ groups. The results also indicated that the muscles are less susceptible to oxidation compared to the rest of the organ samples.
Place, publisher, year, edition, pages
Springer, 2016. Vol. 12, no 2, 19
Thawing effect, Metabolomics, OPLS, Multivariate analysis, Multi-organ
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-117461DOI: 10.1007/s11306-015-0933-1ISI: 000369343900010OAI: oai:DiVA.org:umu-117461DiVA: diva2:907910
Electronic supplementary material The online version of this article (doi:10.1007/s11306-015-0933-1) contains supplementary material, which is available to authorized users.
This research was supported by the Swedish Research Council Grant No. 2011-6044 (to JT), the Biology of Liver and Pancreatic Development and Disease (BOLD) Marie Curie Initial Training Network (MCITN) within EU’s FP7 programme (to TL, JT, KB, FT, SC, CH, TM) and the CNRS and Universite´ Pierre et Marie Curie (to SC, CH), the Institut National de la Sante´ et de la Recherche Me´dicale, INSERM (to SC), the Socie´te´ Francophone du Diabe`te and Emergence UPMC (to CH). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Conflict of interest: JT, TM and TL are shareholders of AcureOmics AB. No financing has been received from this company.2016-03-012016-03-012016-04-05Bibliographically approved