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The cytoplasmic mRNA degradation factor Pat1 is required for rRNA processing
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).ORCID iD: 0000-0003-0482-0543
2016 (English)In: RNA Biology, ISSN 1547-6286, Vol. 13, no 4, 455-465 p.Article in journal (Refereed) Published
Abstract [en]

Pat1 is a key cytoplasmic mRNA degradation factor, the loss of which severely increases mRNA half-lives. Several recent studies have shown that Pat1 can enter the nucleus and can shuttle between the nucleus and the cytoplasm. As a result, many nuclear roles have been proposed for Pat1. In this study, we analyzed four previously suggested nuclear roles of Pat1 and show that Pat1 is not required for efficient pre-mRNA splicing or pre-mRNA decay in yeast. However, lack of Pat1 results in accumulation of pre-rRNA processing intermediates. Intriguingly, we identified a novel genetic relationship between Pat1 and the rRNA decay machinery, specifically the exosome and the TRAMP complex. While the pre-rRNA processing intermediates that accumulate in the pat1 deletion mutant are, at least to some extent, recognized as aberrant by the rRNA degradation machinery, it is unlikely that these accumulations are the cause of their synthetic sick relationship. Here, we show that the dysregulation of the levels of mRNAs related to ribosome biogenesis could be the cause of the accumulation of the pre-rRNA processing intermediates.Although our results support a role for Pat1 in transcription, they nevertheless suggest that the primary cause of the dysregulated mRNA levels is most likely due to Pat1’s role in mRNA decapping and mRNA degradation.

Place, publisher, year, edition, pages
Taylor & Francis, 2016. Vol. 13, no 4, 455-465 p.
Keyword [en]
Lsm1-7, mRNA decapping, Pat1, ribosome biogenesis, rRNA, Rrp6, transcription, Trf4
National Category
Biochemistry and Molecular Biology
Research subject
Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-118072DOI: 10.1080/15476286.2016.1154253ISI: 000373943500008PubMedID: 26918764OAI: oai:DiVA.org:umu-118072DiVA: diva2:911357
Funder
Swedish Research Council, 621-2010-4602Carl Tryggers foundation , 11:330
Available from: 2016-03-11 Created: 2016-03-11 Last updated: 2016-06-10Bibliographically approved

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Muppavarapu, MridulaHuch, SusanneNissan, Tracy
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