Use of two-dimensional protein pattern analysis for the characterization of Arabidopsis thaliana mutants.
1994 (English)In: Planta, ISSN 0032-0935, E-ISSN 1432-2048, Vol. 192, no 4, 557-566 p.Article in journal (Refereed) PublishedText
Total proteins extracted from wild-type plants of Arabidopsis thaliana Heyhn, an etiolated mutant, a de-etiolated mutant and a mutant affected in cotyledon morphology, were analyzed by two-dimensional gel electrophoresis. Computer analysis of two-dimensional gels allowed the characterization of the mutants by a set of proteins showing a differential expression when compared with the wild-type plant grown under the same conditions. The overlap between comparisons of the different mutants with the wild-type allowed the identification of groups of polypeptides which, since their expression is altered in several mutants, might be involved in certain physiological functions. For example, this approach showed a possible involvement of actin in the elongation process. The simultaneous analysis of the two-dimensional protein patterns of different mutants seems, therefore, to be a promising approach to characterize proteins involved in various physiological functions.
Place, publisher, year, edition, pages
1994. Vol. 192, no 4, 557-566 p.
IdentifiersURN: urn:nbn:se:umu:diva-114233OAI: oai:DiVA.org:umu-114233DiVA: diva2:912531
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