A comparison or two-dimensional electrophoresis data with phenotypical traits in Arabidopsis leads to the identification of a mutant (cri1) that accumulates cytokinins
1997 (English)In: Planta, ISSN 0032-0935, E-ISSN 1432-2048, Vol. 202, no 1, 62-69 p.Article in journal (Refereed) PublishedText
Total proteins extracted from developmental mutants of Arabidopsis thaliana (L.) Heyhn, and from wild-type plants cultivated in the presence of various hormones were analyzed by two-dimensional (2-D) gel electrophoresis. Computer analysis of 2-D gels followed by a statistical treatment of data allowed us to build a phenogram that describes the biochemical distances between the different genotypes. Analysis of the 2-D electrophoresis data allowed us to discriminate mutants in agreement with phenotypical and physiological traits. This biochemical analysis helped us to develop a working hypothesis which led us to show that one developmental mutant (cril) overaccumulates cytokinins.
Place, publisher, year, edition, pages
1997. Vol. 202, no 1, 62-69 p.
IdentifiersURN: urn:nbn:se:umu:diva-114234DOI: 10.1007/s004250050103OAI: oai:DiVA.org:umu-114234DiVA: diva2:912532
Times Cited: 32 342016-03-162016-01-152016-03-16