umu.sePublications
Change search
ReferencesLink to record
Permanent link

Direct link
RNA Helicase Important for Listeria monocytogenes Hemolytic Activity and Virulence Factor Expression.
2015 (English)In: Infection and Immunity, ISSN 0019-9567, E-ISSN 1098-5522, Vol. 84, no 1Article in journal (Refereed) Published
Abstract [en]

RNA helicases have been shown to be important for the function of RNA molecules at several levels, although their putative involvement in microbial pathogenesis has remained elusive. We have previously shown that Listeria monocytogenes DExD-box RNA helicases are important for bacterial growth, motility, ribosomal maturation, and rRNA processing. We assessed the importance of the RNA helicase Lmo0866 (here named CshA) for expression of virulence traits. We observed a reduction in hemolytic activity in a strain lacking CshA compared to the wild type. This phenomenon was less evident in strains lacking other RNA helicases. The reduced hemolysis was accompanied by lower expression of major listerial virulence factors in the ΔcshA strain, mainly listeriolysin O, but also to some degree the actin polymerizing factor ActA. Reduced expression of these virulence factors in the strain lacking CshA did not, however, correlate with a decreased level of the virulence regulator PrfA. When combining the ΔcshA knockout with a mutation creating a constitutively active PrfA protein (PrfA*), the effect of the ΔcshA knockout on LLO expression was negated. These data suggest a role for the RNA helicase CshA in posttranslational activation of PrfA. Surprisingly, although the expression of several virulence factors was reduced, the ΔcshA strain did not demonstrate any reduced ability to infect nonphagocytic cells compared to the wild-type strain.

Place, publisher, year, edition, pages
2015. Vol. 84, no 1
National Category
Microbiology
Identifiers
URN: urn:nbn:se:umu:diva-118560DOI: 10.1128/IAI.00849-15PubMedID: 26483402OAI: oai:DiVA.org:umu-118560DiVA: diva2:914041
Available from: 2016-03-23 Created: 2016-03-23 Last updated: 2016-03-23

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
In the same journal
Infection and Immunity
Microbiology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 21 hits
ReferencesLink to record
Permanent link

Direct link