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Minimal selective concentrations of tetracycline in complex aquatic bacterial biofilms
Umeå University, Faculty of Science and Technology, Department of Chemistry.
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2016 (English)In: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 553, 587-595 p.Article in journal (Refereed) PublishedText
Abstract [en]

Selection pressure generated by antibiotics released into the environment could enrich for antibiotic resistance genes and antibiotic resistant bacteria, thereby increasing the risk for transmission to humans and animals. Tetracyclines comprise an antibiotic class of great importance to both human and animal health. Accordingly, residues of tetracycline are commonly detected in aquatic environments. To assess if tetracycline pollution in aquatic environments promotes development of resistance, we determined minimal selective concentrations (MSCs) in biofilms of complex aquatic bacterial communities using both phenotypic and genotypic assays. Tetracycline significantly increased the relative abundance of resistant bacteria at 10 μg/L, while specific tet genes (tetA and tetG) increased significantly at the lowest concentration tested (1 μg/L). Taxonomic composition of the biofilm communities was altered with increasing tetracycline concentrations. Metagenomic analysis revealed a concurrent increase of several tet genes and a range of other genes providing resistance to different classes of antibiotics (e.g. cmlA, floR, sul1, and mphA), indicating potential for co-selection. Consequently, MSCs for the tet genes of ≤ 1 μg/L suggests that current exposure levels in e.g. sewage treatment plants could be sufficient to promote resistance. The methodology used here to assess MSCs could be applied in risk assessment of other antibiotics as well.

Place, publisher, year, edition, pages
Elsevier, 2016. Vol. 553, 587-595 p.
Keyword [en]
Minimal selective concentration, Antibiotic resistance, Risk assessment, Antibiotic contaminants, Environmental emission limits
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:umu:diva-118609DOI: 10.1016/j.scitotenv.2016.02.103ISI: 000373220700059OAI: oai:DiVA.org:umu-118609DiVA: diva2:914435
Available from: 2016-03-24 Created: 2016-03-24 Last updated: 2016-08-10Bibliographically approved

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Östman, MarcusTysklind, Mats
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