Identification and Characterization of IgdE, a Novel IgG-degrading Protease of Streptococcus suis with Unique Specificity for Porcine IgG.
2016 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 291, no 15, 7915-7925 p.Article in journal (Refereed) Published
Streptococcus suis is a major endemic pathogen of pigs causing meningitis, arthritis, and other diseases. Zoonotic S. suis infections are emerging in humans causing similar pathologies as well as severe conditions such as toxic shock-like syndrome. Recently, we discovered an IdeS family protease of S. suis that exclusively cleaves porcine IgM and represents the first virulence factor described, linking S. suis to pigs as their natural host. Here we report the identification and characterization of a novel, unrelated protease of S. suis that exclusively targets porcine IgG. This enzyme, designated IgdE for immunoglobulin G-degrading enzyme of S. suis, is a cysteine protease distinct from previous characterized streptococcal immunoglobulin degrading proteases of the IdeS family and mediates efficient cleavage of the hinge region of porcine IgG with a high degree of specificity. The findings that all S. suis strains investigated possess the IgG proteolytic activity and that piglet serum samples contain specific antibodies against IgdE strongly indicate that the protease is expressed in vivo during infection and represents a novel and putative important bacterial virulence/colonization determinant, and a thus potential therapeutic target.
Place, publisher, year, edition, pages
2016. Vol. 291, no 15, 7915-7925 p.
antibody, cysteine protease, immunoglobulin G (IgG), proteolytic enzyme, Streptococcus, substrate specificity
Other Natural Sciences Other Veterinary Science Microbiology in the medical area
IdentifiersURN: urn:nbn:se:umu:diva-118732DOI: 10.1074/jbc.M115.711440ISI: 000374056700014PubMedID: 26861873OAI: oai:DiVA.org:umu-118732DiVA: diva2:915828