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NMR-based metabolite profiling of human milk: A pilot study of methods for investigating compositional changes during lactation
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Umeå universitet, Medicinska fakulteten, Institutionen för klinisk vetenskap, Pediatrik.
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk kemi och biofysik.
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2016 (Engelska)Ingår i: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 469, nr 3, s. 626-632Artikel i tidskrift (Refereegranskat) Published
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Abstract [en]

Low-molecular-weight metabolites in human milk are gaining increasing interest in studies of infant nutrition. In the present study, the milk metabolome from a single mother was explored at different stages of lactation. Metabolites were extracted from sample aliquots using either methanol water (MeOH/H2O) extraction or ultrafiltration. Nuclear magnetic resonance (NMR) spectroscopy was used for metabolite identification and quantification, and multi- and univariate statistical data analyses were used to detect changes over time of lactation. Compared to MeOH/H2O extraction, ultrafiltration more efficiently reduced the interference from lipid and protein resonances, thereby enabling the identification and quantification of 36 metabolites. The human milk metabolomes at the early (9-24 days after delivery) and late (31-87 days after delivery) stages of lactation were distinctly different according to multi- and univariate statistics. The late lactation stage was characterized by significantly elevated concentrations of lactose, choline, alanine, glutamate, and glutamine, as well as by reduced levels of citrate, phosphocholine, glycerophosphocholine, and N-acetylglucosamine. Our results indicate that there are significant compositional changes of the human milk metabolome also in different phases of the matured lactation stage. These findings complement temporal studies on the colostrum and transitional metabolome in providing a better understanding of the nutritional variations received by an infant.

Ort, förlag, år, upplaga, sidor
2016. Vol. 469, nr 3, s. 626-632
Nyckelord [en]
Human milk, Lactation, NMR, Metabolomics, Metabonomics
Nationell ämneskategori
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
Identifikatorer
URN: urn:nbn:se:umu:diva-117401DOI: 10.1016/j.bbrc.2015.11.114ISI: 000369352800046PubMedID: 26655810OAI: oai:DiVA.org:umu-117401DiVA, id: diva2:916958
Tillgänglig från: 2016-04-05 Skapad: 2016-02-29 Senast uppdaterad: 2018-06-07Bibliografiskt granskad

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Wu, JunfangDomellöf, MagnusÖhman, AndersNording, Malin L.

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Wu, JunfangDomellöf, MagnusÖhman, AndersNording, Malin L.
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Kemiska institutionenPediatrikInstitutionen för medicinsk kemi och biofysikKlinisk neurovetenskap
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Biochemical and Biophysical Research Communications - BBRC
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)

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