Change search
ReferencesLink to record
Permanent link

Direct link
GC/MS analysis of the rat urine for metabonomic research
Show others and affiliations
2007 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 854, no 1-2, 20-25 p.Article in journal (Refereed) PublishedText
Abstract [en]

In this paper, an optimized protocol was established and validated for the metabonomic profiling in rat urine using GC/MS. The urine samples were extracted by methanol after treatment with urease to remove excessive urea, then the resulted supernatant was dried, methoximated, trimethylsilylated, and analyzed by GC/MS. Forty-nine endogenous metabolites were separated and identified in GC/MS chromatogram, of which 26 identified compounds were selected for quantitative analysis to evaluate the linearity, precision, and sensitivity of the method. It showed good linearity between mass spectrometry responses and relative concentrations of the 26 endogenous compounds over the range from 0.063 to 1.000(v/v, urine/urine+ water) and satisfactory reproducibility with intra-day and inter-days precision values all below 15%. The metabonomic profiling method based on GC/MS was successfully applied to urine samples from hyperlipidemia model rats. Obviously, separated clustering of model rats and the control rats were shown by principal components analysis (PCA); time-dependent metabonomic modification was detected as well. It was suggested that metabonomic profiling based on GC/MS be a robust method for urine samples.

Place, publisher, year, edition, pages
Amsterdam: Elsevier, 2007. Vol. 854, no 1-2, 20-25 p.
Keyword [en]
metabonomics, urine, GC/MS
National Category
Biochemistry and Molecular Biology Analytical Chemistry
URN: urn:nbn:se:umu:diva-118205DOI: 10.1016/j.jchromb.2007.03.048ISI: 000248161900004PubMedID: 17526439OAI: diva2:918851
Available from: 2016-04-12 Created: 2016-03-14 Last updated: 2016-04-12Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Jiye, A
By organisation
Clinical chemistry
In the same journal
Journal of chromatography. B
Biochemistry and Molecular BiologyAnalytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 5 hits
ReferencesLink to record
Permanent link

Direct link