Application of a metabolomic method combining one-dimensional and two-dimensional gas chromatography-time-of-flight/mass spectrometry to metabolic phenotyping of natural variants in rice
2007 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 855, no 1, 71-79 p.Article in journal (Refereed) PublishedText
We have developed a comprehensive method combining analytical techniques of one-dimensional (I D) and two-dimensional (GC x GC) gas chromatography-time-of-flight (TOF)-mass spectrometry. This method was applied to the metabolic phenotyping of natural variants in rice for the 68 world rice core collection (WRC) and two other varieties. Ten metabolites were selected as metabolite representatives, and the selected ion current of each metabolite peak obtained from both techniques were statistically compared. Our method of combining I D- and GC x GC-TOF/MS is useful for the metabolic phenotyping of natural variants in rice for further studies in breeding programs.
Place, publisher, year, edition, pages
Amsterdam: Elsevier, 2007. Vol. 855, no 1, 71-79 p.
rice core collection, GC x GC, two-dimensional chromatography, gas chromatography, metabolic phenotyping
Biochemistry and Molecular Biology Chemical Sciences
IdentifiersURN: urn:nbn:se:umu:diva-118197DOI: 10.1016/j.jchromb.2007.05.002ISI: 000248893000011PubMedID: 17556050OAI: oai:DiVA.org:umu-118197DiVA: diva2:920529
31st Annual Meeting of the Japanese Society for Biomedical Mass Spectrometry, SEP 28-29, 2006, Nagoya, JAPAN