Change search
ReferencesLink to record
Permanent link

Direct link
Cathepsin K Contributes to Cavitation and Collagen Turnover in Pulmonary Tuberculosis
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology).
Show others and affiliations
2016 (English)In: Journal of Infectious Diseases, ISSN 0022-1899, E-ISSN 1537-6613, Vol. 213, no 4, 618-627 p.Article in journal (Refereed) PublishedText
Abstract [en]

Cavitation in tuberculosis enables highly efficient person-to-person aerosol transmission. We performed transcriptomics in the rabbit cavitary tuberculosis model. Among 17 318 transcripts, we identified 22 upregulated proteases. Five type I collagenases were overrepresented: cathepsin K (CTSK), mast cell chymase-1 (CMA1), matrix metalloproteinase 1 (MMP-1), MMP-13, and MMP-14. Studies of collagen turnover markers, specifically, collagen type I C-terminal propeptide (CICP), urinary deoxypyridinoline (DPD), and urinary helical peptide, revealed that cavitation in tuberculosis leads to both type I collagen destruction and synthesis and that proteases other than MMP-1, MMP-13, and MMP-14 are involved, suggesting a key role for CTSK. We confirmed the importance of CTSK upregulation in human lung specimens, using immunohistochemical analysis, which revealed perigranulomatous staining for CTSK, and we showed that CTSK levels were increased in the serum of patients with tuberculosis, compared with those in controls (3.3 vs 0.3 ng/mL; P = .005).

Place, publisher, year, edition, pages
Oxford University Press, 2016. Vol. 213, no 4, 618-627 p.
Keyword [en]
tuberculosis, cathepsin K, rabbit, collagen, collagenolysis, RNAseq
National Category
Microbiology Immunology
URN: urn:nbn:se:umu:diva-119672DOI: 10.1093/infdis/jiv458ISI: 000372437700019PubMedID: 26416658OAI: diva2:923047
Available from: 2016-04-25 Created: 2016-04-25 Last updated: 2016-04-25Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Larsson, Christer
By organisation
Department of Molecular Biology (Faculty of Science and Technology)
In the same journal
Journal of Infectious Diseases

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 4 hits
ReferencesLink to record
Permanent link

Direct link