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A cell wall damage response mediated by a sensor kinase/response regulator pair enables beta-lactam tolerance
Umeå universitet, Medicinska fakulteten, Umeå Centre for Microbial Research (UCMR). Umeå universitet, Medicinska fakulteten, Institutionen för molekylärbiologi (Medicinska fakulteten). Umeå universitet, Medicinska fakulteten, Molekylär Infektionsmedicin, Sverige (MIMS).
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2016 (Engelska)Ingår i: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 113, nr 2, s. 404-409Artikel i tidskrift (Refereegranskat) Published
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Abstract [en]

The bacterial cell wall is critical for maintenance of cell shape and survival. Following exposure to antibiotics that target enzymes required for cell wall synthesis, bacteria typically lyse. Although several cell envelope stress response systems have been well described, there is little knowledge of systems that modulate cell wall synthesis in response to cell wall damage, particularly in Gram-negative bacteria. Here we describe WigK/WigR, a histidine kinase/response regulator pair that enables Vibrio cholerae, the cholera pathogen, to survive exposure to antibiotics targeting cell wall synthesis in vitro and during infection. Unlike wild-type V. cholerae, mutants lacking wigR fail to recover following exposure to cell-wall-acting antibiotics, and they exhibit a drastically increased cell diameter in the absence of such antibiotics. Conversely, overexpression of wigR leads to cell slimming. Overexpression of activated WigR also results in increased expression of the full set of cell wall synthesis genes and to elevated cell wall content. WigKR-dependent expression of cell wall synthesis genes is induced by various cell-wall-acting antibiotics as well as by overexpression of an endogenous cell wall hydrolase. Thus, WigKR appears to monitor cell wall integrity and to enhance the capacity for increased cell wall production in response to damage. Taken together, these findings implicate WigKR as a regulator of cell wall synthesis that controls cell wall homeostasis in response to antibiotics and likely during normal growth as well.

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2016. Vol. 113, nr 2, s. 404-409
Nyckelord [en]
peptidoglycan, stress response, antibiotic tolerance, two component system, cell envelope
Nationell ämneskategori
Cell- och molekylärbiologi Mikrobiologi
Identifikatorer
URN: urn:nbn:se:umu:diva-114884DOI: 10.1073/pnas.1520333113ISI: 000367881500053PubMedID: 26712007OAI: oai:DiVA.org:umu-114884DiVA, id: diva2:923804
Forskningsfinansiär
Knut och Alice Wallenbergs StiftelseVetenskapsrådetKempestiftelsernaTillgänglig från: 2016-04-27 Skapad: 2016-01-29 Senast uppdaterad: 2018-06-07Bibliografiskt granskad

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Alvarez, LauraCava, Felipe

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Alvarez, LauraCava, Felipe
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Umeå Centre for Microbial Research (UCMR)Institutionen för molekylärbiologi (Medicinska fakulteten)Molekylär Infektionsmedicin, Sverige (MIMS)
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Proceedings of the National Academy of Sciences of the United States of America
Cell- och molekylärbiologiMikrobiologi

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