Sulfate reduction potential in sediments in the Norilsk mining area, northern Siberia
2005 (English)In: Geomicrobiology Journal, ISSN 0149-0451, E-ISSN 1521-0529, Vol. 22, no 1-2, 11-25 p.Article in journal (Refereed) PublishedText
The purpose of this study was to characterize the distribution and activity of sulfate-reducing bacteria in tailings and sediments impacted by effluents from mining and smelting operations in the Norilsk area in northern Siberia. The Norilsk mining complex involves three smelter operations, a hydrometallurgical plant, and extensive tailings areas located in the permafrost zone. Sulfate reduction rates measured with a (35)SO(4)(2-) tracer technique under various in-situ conditions ranged from 0.05 to 30 nmol S cm(-3) day(-1). Acetate and glucose addition greatly stimulated sulfate reduction, whereas lactate had less effect. The most pronounced stimulation of sulfate reduction (6.5-fold) was observed with phosphate amendment. Most-probable-number (MPN) counts of sulfate-reducing bacteria in media with glucose, ethanol, lactate, and acetate as electron donors were generally highest at around 10(7) cells ml(-1). The actual MPN counts varied with the sample, electron donor, and incubation conditions (pH 7.2 vs. pH 3.5; 28 degrees C vs. 4 degrees C). Enrichment cultures of sulfate-reducing bacteria were established from a sample that showed the highest rate of sulfate reduction. After multiple serial transfers, the dominant sulfate-reducers were identified by fluorescence in situ hybridization using genus and group-specific 16S rRNA-targeted oligonucleotide probes. Desulfobulbus spp. prevailed in ethanol and lactate enrichments and the Desulfosarcina-Desulfococcus group dominated in acetate and benzoate enrichments. Psychrophilic Desulfotalea-Desulfofustis and moderately psychrophilic Desulforhopalus spp. were identified in enrichments incubated at 4 degrees C, but they were also found in mesophilic enrichments.
Place, publisher, year, edition, pages
Philadelphia: Taylor & Francis, 2005. Vol. 22, no 1-2, 11-25 p.
arctic sediments, enrichment of sulfate reducers, FISH, mine tailings, stable isotope fractionation, sulfate reduction
Environmental Sciences Geosciences, Multidisciplinary
IdentifiersURN: urn:nbn:se:umu:diva-120450DOI: 10.1080/01490450590922523ISI: 000228319100002OAI: oai:DiVA.org:umu-120450DiVA: diva2:929001