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Characterisation of Schizosaccharomyces pombe alpha-actinin
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
2016 (English)In: PeerJ, ISSN 2167-8359, E-ISSN 2167-8359, Vol. 4, e1858Article in journal (Refereed) Published
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Abstract [en]

The actin cytoskeleton plays a fundamental role in eukaryotic cells. Its reorganization is regulated by a plethora of actin-modulating proteins, such as a-actinin. In higher organisms, alpha-actinin is characterized by the presence of three distinct structural domains: an N-terminal actin-binding domain and a C-terminal region with EF-hand motif separated by a central rod domain with four spectrin repeats. Sequence analysis has revealed that the central rod domain of alpha-actinin from the fission yeast Schizosaccharomyces pombe consists of only two spectrin repeats. To obtain a firmer understanding of the structure and function of this unconventional alpha-actinin, we have cloned and characterized each structural domain. Our results show that this alpha-actinin isoform is capable of forming dimers and that the rod domain is required for this. However, its actin-binding and cross-linking activity appears less efficient compared to conventional alpha-actinins. The solved crystal structure of the actin-binding domain indicates that the closed state is stabilised by hydrogen bonds and a salt bridge not present in other a-actinins, which may reduce the affinity for actin.

Place, publisher, year, edition, pages
2016. Vol. 4, e1858
Keyword [en]
Spectrin repeat, Actin-binding protein, alpha-actinin, Schizosaccharomyces pombe
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:umu:diva-120364DOI: 10.7717/peerj.1858ISI: 000374159200008PubMedID: 27069798OAI: oai:DiVA.org:umu-120364DiVA: diva2:929136
Funder
Carl Tryggers foundation
Available from: 2016-05-17 Created: 2016-05-16 Last updated: 2017-11-30Bibliographically approved

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Sandblad, LindaPersson, KarinaBackman, Lars
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