umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Regulation of assembly and cell surface dynamics of caveolae
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics. (Richard Lundmark)ORCID iD: 0000-0002-0309-0000
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

A typical mammalian cell plasma membrane displays a high level of plasticity counter balanced with stability. The plasma membrane show various kinds of invaginations to meet physiological demands of the cells such as nutrient uptake, receptor signaling etc. An example of one such invagination observed in many cell types is “Caveola”. Caveolae are bulb shaped invaginations of the plasma membrane enriched in sphingolipids and cholesterol. Each individual caveola is in equilibrium with multi caveolar assemblies and exhibits various dynamics ranging from stable association with cell surface to the “kiss and run” type and internalized caveolae called cavicle. The principle protein components of caveolae involve caveolin1, 2 and 3, cavin 1, 2, 3 and 4 along with EHD2, PACSIN2 and dynamin. Caveolin1 acts as the hallmark of caveolae, whereas caveolin3 and cavin4 are limited to muscle cells. Caveolae are appreciated as important plasma membrane structures in maintaining cellular homeostasis of many cell types. Its dysfunction is associated with several human diseases such as cancer, vascular diseases, lipid and muscular dystrophies.

This thesis aims to understand the assembly of caveolae and the molecular machineries involved in the regulation of caveolae dynamics. In particular, we have focused on the mechanism of cavin coat assembly, the influence of cavin3 in the regulation of caveolae dynamics, along with the mechanistic cycle of EHD2.

Cavins form characteristic striations around caveolae, and in this work we showed that the N-terminus of cavin3 interacts with the trimeric N-terminus of cavin1 in competition with the N-terminus of cavin2 to form cavin sub-complex. We also observed that cavin3 interacts with caveolin1 in a cholesterol dependent manner and cavin3 may promote scission by acting as a positive regulator of caveolae dynamics, opposite to the cellular function of EHD2. The stringent roles of cavin3 and EHD2 control the equilibrium between stably cell surface associated caveolae and the “kiss and run” type of caveolae, undergoing rounds of fission and fusion. Our results demonstrated the molecular composition of the caveolae coat at a domain level with the stoichiometry of cavin sub-complexes. We also showed the function of cavin3 in the regulation of caveolae dynamics at the plasma membrane. Previous work from our lab showed that EHD2 is a dimeric ATPase localised to the caveolae neck and confines caveolae to the cell surface. In the present study, we showed that EHD2 oligomerized in an open conformation stabilized by ATP and in a G-domain loop dependent manner. The oligomerization of EHD2 in cells is finely tuned by the N-terminal region and the C-terminal EH domain, where both of these regions act as negative regulators of membrane binding. Our results showed the stringent regulation of EHD2 oligomerization and its importance with respect to various statuses and/or function of caveolae.

In summary, the current study provides a novel insight into the assembly of cavin coat and protein machineries involved in the regulation of caveolae dynamics. In addition, it also contributes to the understanding of the molecular mechanism of ATPase activity of EHD2. 

Place, publisher, year, edition, pages
Umeå: Umeå University , 2016. , 52 p.
Series
Umeå University medical dissertations, ISSN 0346-6612 ; 1816
National Category
Biochemistry and Molecular Biology
Research subject
Medical Biochemistry
Identifiers
URN: urn:nbn:se:umu:diva-120753ISBN: 978-91-7601-496-7 (print)OAI: oai:DiVA.org:umu-120753DiVA: diva2:929812
Public defence
2016-06-15, N450, Naturvetarhuset, Umeå University, Umeå, 09:00 (English)
Opponent
Supervisors
Available from: 2016-05-24 Created: 2016-05-20 Last updated: 2016-05-26Bibliographically approved
List of papers
1. Cavin3 interacts with cavin1 and caveolin1 to increase surface dynamics of caveolae
Open this publication in new window or tab >>Cavin3 interacts with cavin1 and caveolin1 to increase surface dynamics of caveolae
Show others...
2015 (English)In: Journal of Cell Science, ISSN 0021-9533, E-ISSN 1477-9137, Vol. 128, no 5, 979-991 p.Article in journal (Refereed) Published
Abstract [en]

Caveolae are invaginations of the cell surface thought to regulate membrane tension, signalling, adhesion and lipid homeostasis due to their dynamic behaviour ranging from stable surface association to dynamic rounds of fission and fusion with the plasma membrane. The caveolae coat is generated by oligomerisation of the membrane protein caveolin and the family of cavin proteins. Here, we show that cavin3 is targeted to caveolae by cavin1 where it interacts with the scaffolding domain of caveolin1 and promote caveolae dynamics. We found that the N-terminal region of cavin3 binds a trimer of the cavin1 N-terminus in competition with a homologous cavin2 region, showing that the cavins form distinct subcomplexes via their N-terminal regions. Our data shows that cavin3 is enriched at deeply invaginated caveolae and that loss of cavin3 in cells results in an increase of stable caveolae and a decrease of caveolae with short duration time at the membrane. We propose that cavin3 is recruited to the caveolae coat by cavin1 to interact with caveolin1 and regulate the duration time of caveolae at the plasma membrane.

Keyword
Cavin1, Cavin3, Caveolin1, Caveolae, EHD2
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-92497 (URN)10.1242/jcs.161463 (DOI)000350569900014 ()
Available from: 2014-08-27 Created: 2014-08-27 Last updated: 2017-12-05Bibliographically approved
2. Structural arrangement of membrane-bound cavin
Open this publication in new window or tab >>Structural arrangement of membrane-bound cavin
Show others...
(English)Manuscript (preprint) (Other academic)
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-120757 (URN)
Available from: 2016-05-20 Created: 2016-05-20 Last updated: 2016-05-20
3. TP driven stabilization of a membrane bound open confirmation of the ATPase EHD2 restrains caveolae dynamics
Open this publication in new window or tab >>TP driven stabilization of a membrane bound open confirmation of the ATPase EHD2 restrains caveolae dynamics
Show others...
(English)Manuscript (preprint) (Other academic)
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-120758 (URN)
Available from: 2016-05-20 Created: 2016-05-20 Last updated: 2016-05-20

Open Access in DiVA

fulltext(20551 kB)837 downloads
File information
File name FULLTEXT01.pdfFile size 20551 kBChecksum SHA-512
76921c3cdf574d6508dd073b954784b1ba4e799b3d719a1c570ff11f25bb609a83d8a0cbba96c254f74398f516ce6d8cfedbedf16dbc366d3a2c3f05a90d4e3e
Type fulltextMimetype application/pdf
spikblad(100 kB)28 downloads
File information
File name SPIKBLAD01.pdfFile size 100 kBChecksum SHA-512
4e42a5f074337344657f7b8f444e1620ad7564b20119b781974261468da369ce0bdccb352e503c8d892c8477cf32a1641bc24f1f1f099493fd9fbd96676f1a47
Type spikbladMimetype application/pdf

Search in DiVA

By author/editor
Mohan, Jagan
By organisation
Department of Medical Biochemistry and Biophysics
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
Total: 837 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 526 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf