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Vibrio cholerae hemagglutinin/protease (HA/protease) causes morphological changes in cultured epithelial cells and perturbs their paracellular barrier function.
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology).
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1996 (English)In: Microbial Pathogenesis, ISSN 0882-4010, E-ISSN 1096-1208, Vol. 21, no 2Article in journal (Refereed) Published
Abstract [en]

In this report, we describe the cytotoxic activity of the cholera hemagglutinin/protease (HA/protease). A concentrated protein sample from the 37 degrees C overnight culture supernatant of CVD110, a delta ctxA, delta zot, delta Ace and hlyA::(ctxB mer) mutant of El Tor biotype Ogawa serotype strain E7946 caused morphological changes in cultured MDCK-I epithelial cells and altered their arrangement of filamentous actin (F-actin) and Zonula occludens-associated protein ZO-1. The drastic morphological changes can be inhibited by Zincov, a specific bacterial metalloprotease inhibitor. The cytotoxic fractions of the sample after FPLC gelfiltration fractionation showed two visible protein bands with molecular weights of approximately 34- and 32 kDa. Microsequencing of these two proteins revealed that they were the cholera HA/protease.

Place, publisher, year, edition, pages
1996. Vol. 21, no 2
National Category
Microbiology
Identifiers
URN: urn:nbn:se:umu:diva-121659DOI: 10.1006/mpat.1996.0047PubMedID: 8844654OAI: oai:DiVA.org:umu-121659DiVA: diva2:933339
Available from: 2016-06-03 Created: 2016-06-03 Last updated: 2016-06-03

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