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The anterior chamber of the eye is a transplantation site that supports and enables visualisation of beta cell development in mice
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2016 (English)In: Diabetologia, ISSN 0012-186X, E-ISSN 1432-0428, Vol. 59, no 5, 1007-1011 p.Article in journal (Refereed) PublishedText
Abstract [en]

In vivo imaging of the developing pancreas is challenging due to the inaccessibility of the tissue. To circumvent this, on embryonic day 10.5 (E10.5) we transplanted a mouse developing pancreatic bud into the anterior chamber of the eye (ACE) to determine whether the eye is a useful transplant site to support pancreas development. We transplanted an E10.5 dorsal pancreatic bud into the ACE of a syngeneic recipient mouse. Using a mouse insulin promoter-green fluorescent protein (MIP-GFP) mouse as the tissue donor, we non-invasively imaged the pancreatic bud as it develops at single beta cell resolution across time. The transplanted pancreatic bud rapidly engrafts and vascularises when transplanted into the ACE. The pancreatic progenitor cells differentiate into exocrine and endocrine cells, including cells expressing insulin, glucagon and somatostatin. The morphology of the transplanted pancreatic bud resembles that of the native developing pancreas. Beta cells within the transplanted pancreatic bud respond to glucose in a manner similar to that of native fetal beta cells and superior to that of in vitro developed beta cells. Unlike in vitro grown pancreatic explants, pancreatic tissue developing in the ACE is vascularised, providing the developing pancreatic tissue with a milieu resembling the native situation. Altogether, we show that the ACE is able to support growth, differentiation and function of a developing pancreatic bud across time in vivo.

Place, publisher, year, edition, pages
2016. Vol. 59, no 5, 1007-1011 p.
Keyword [en]
Beta cells, Eye transplantation, In vivo imaging, Pancreas development, Pancreatic islets
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-120087DOI: 10.1007/s00125-016-3883-xISI: 000373993300017PubMedID: 26847769OAI: oai:DiVA.org:umu-120087DiVA: diva2:936045
Available from: 2016-06-13 Created: 2016-05-09 Last updated: 2016-06-13Bibliographically approved

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Edlund, Helena
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Umeå Centre for Molecular Medicine (UCMM)
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