Change search
ReferencesLink to record
Permanent link

Direct link
Analysis of Bacterial Cell Surface Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
2016 (English)In: Bacterial Cell Wall Homeostasis: Methods and Protocols / [ed] Hong, Hee-Jeon, New York, NY, 2016, 215-223 p.Chapter in book (Other academic)Text
Abstract [en]

This chapter describes a method for measuring the average surface chemical composition with respect to lipids, polysaccharides, and peptides (protein + peptidoglycan) for the outer part of the bacterial cell wall. Bacterial cultures grown over night are washed with a buffer or saline at controlled pH. The analysis is done on fast-frozen bacterial cell pellets obtained after centrifugation, and the analysis requires access to X-ray photoelectron spectroscopy instrumentation that can perform analyses at cryogenic temperatures (for example using liquid nitrogen). The method can be used to monitor changes in the cell wall composition following environmental stimuli or genetic mutations. The data obtained originate from the outermost part of the cell wall. Thus, it is expected that for gram-negative bacteria only the outer membrane and part of the periplasmic peptidoglycan layer is probed during analysis, and for gram-positive bacteria only the top nanometers of the peptidoglycan layer of the cell wall is monitored.

Place, publisher, year, edition, pages
New York, NY, 2016. 215-223 p.
Keyword [en]
X-ray photoelectron spectroscopy Cell wall composition C 1s spectra Bacterial cells
National Category
Chemical Sciences
URN: urn:nbn:se:umu:diva-122430DOI: 10.1007/978-1-4939-3676-2_16ISBN: 978-1-4939-3676-2OAI: diva2:938851
Available from: 2016-06-17 Created: 2016-06-17 Last updated: 2016-06-17

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Ramstedt, MadeleineShchukarev, Andrey
By organisation
Department of Chemistry
Chemical Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 24 hits
ReferencesLink to record
Permanent link

Direct link