Induction of apoptosis and G2/M arrest by 2-Methoxyestradiol in human cervical cancer HeLaS3 cells
2004 (English)In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 24, no 2B, 873-880 p.Article in journal (Refereed) PublishedText
Background: It has been demonstrated that 2-Methoxyestradiol (2-ME), one of the estrogen metabolites, induces apoptosis in many different tumor cell lines. In the present study, the effects of 2-ME on human cervical cancer HeLaS3 cells and on normal cervical epithelial cells were evaluated.
Materials and Methods: Acridine orange staining, DNA fragmentation arrays and flow cytometry were used to measure the apoptosis and cell cycle progression. In addition, the effect of 2-ME on expression of iNOS was measured by Western blot.
Results: 2-ME inhibited the growth of HeLaS3 cells. This growth inhibition was accompanied by apoptosis and G(2)/M cell cycle arrest. 2-ME increased the expression of iNOS in parallel with apoptosis. Moreover, apoptosis was prevented by the iNOS inhibitor 1400W. 2-ME treatment resulted in a slight increase of the G(2)/M population, but no apoptosis, in normal cervical epithelial cells. There was no synergetic effect between E? and 2-ME.
Conclusion: 2-ME induced apoptosis via the iNOS pathway and caused G(2)/M cell cycle arrest in human cervical cancer HeLaS3 cells, but showed only slight effects on normal cervical epithelial cells. These data suggest that 2-ME might be an adjuvant agent in the treatment of cervical cancer.
Place, publisher, year, edition, pages
Athens: International institute for anticancer research , 2004. Vol. 24, no 2B, 873-880 p.
cervical cells, HeLaS3 cells, 2-methoxyestradiol, apoptosis, cell cycle
Cancer and Oncology
IdentifiersURN: urn:nbn:se:umu:diva-122172ISI: 000221385000050PubMedID: 15161040OAI: oai:DiVA.org:umu-122172DiVA: diva2:940019