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Polarisation of type III translocation by Pseudomonas aeruginosa requires PcrG, PcrV and PopN
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
2004 (English)In: Microbial Pathogenesis, ISSN 0882-4010, E-ISSN 1096-1208, Vol. 37, no 6, 313-322 p.Article in journal (Refereed) PublishedText
Abstract [en]

Type III secretion (TTS) mediated translocation of exoenzymes is a key virulence strategy utilised by the opportunistic pathogen Pseudomonas aeruginosa to deliver exoenzyme effectors into the eukaryotic cell. We have previously shown that type III mediated translocation is a contact dependent process, which requires the secreted translocator proteins PerV, PopB and PopD. To further analyse this mechanism, HeLa cells were infected with the wild-type strain PAK as well as isogenic pcrV, popB, popD, pcrG and popN mutants. In the presence of eukaryotic cells, expression of exoenzyme S (ExoS) increased. When cells were infected with the wild-type strain PAK no ExoS was detected in the tissue culture medium. This confirms that ExoS translocation by P. aeruginosa occurs by a polarised mechanism. In contrast, high levels of ExoS were recovered in the tissue Culture medium when cells were infected with pcrG, pcrV and popN mutants. Additionally, ExoS expression levels were higher for these mutants regardless of inducing conditions. This suggests that PcrG, PcrV and PopN are involved in negative regulation of ExoS expression and secretion, and are required to ensure polarised delivery of effectors into target cells.

Place, publisher, year, edition, pages
London: Academic Press, 2004. Vol. 37, no 6, 313-322 p.
Keyword [en]
Pseudomonas aeruginosa, type III secretion, ExoS, PcrV, PcrG, PopN
National Category
Immunology in the medical area Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-122140DOI: 10.1016/j.micpath.2004.10.005ISI: 000226441500005PubMedID: 15619427OAI: oai:DiVA.org:umu-122140DiVA: diva2:942240
Available from: 2016-06-23 Created: 2016-06-15 Last updated: 2016-06-23Bibliographically approved

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Sundin, CharlottaBröms, Jeanette EForsberg, Åke
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Microbial Pathogenesis
Immunology in the medical areaMicrobiology in the medical area

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