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Biogenesis of water splitting by photosystem II during de-etiolation of barley (Hordeum vulgare L.)
Umeå University, Faculty of Science and Technology, Department of Chemistry. Centre for Organelle Research, Faculty of Science and Technology, University of Stavanger, Stavanger, Norway.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
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2016 (English)In: Plant, Cell and Environment, ISSN 0140-7791, E-ISSN 1365-3040, Vol. 39, no 7, 1524-1536 p.Article in journal (Refereed) Published
Abstract [en]

Etioplasts lack thylakoid membranes and photosystem complexes. Light triggers differentiation of etioplasts into mature chloroplasts, and photosystem complexes assemble in parallel with thylakoid membrane development. Plastids isolated at various time points of de-etiolation are ideal to study the kinetic biogenesis of photosystem complexes during chloroplast development. Here, we investigated the chronology of photosystem II (PSII) biogenesis by monitoring assembly status of chlorophyll-binding protein complexes and development of water splitting via O2 production in plastids (etiochloroplasts) isolated during de-etiolation of barley (Hordeum vulgare L.). Assembly of PSII monomers, dimers and complexes binding outer light-harvesting antenna [PSII-light-harvesting complex II (LHCII) supercomplexes] was identified after 1, 2 and 4 h of de-etiolation, respectively. Water splitting was detected in parallel with assembly of PSII monomers, and its development correlated with an increase of bound Mn in the samples. After 4 h of de-etiolation, etiochloroplasts revealed the same water-splitting efficiency as mature chloroplasts. We conclude that the capability of PSII to split water during de-etiolation precedes assembly of the PSII-LHCII supercomplexes. Taken together, data show a rapid establishment of water-splitting activity during etioplast-to-chloroplast transition and emphasize that assembly of the functional water-splitting site of PSII is not the rate-limiting step in the formation of photoactive thylakoid membranes.

Place, publisher, year, edition, pages
John Wiley & Sons, 2016. Vol. 39, no 7, 1524-1536 p.
Keyword [en]
chloroplast biogenesis, oxygen evolution, oxygen-evolving complex, photosystem II assembly
National Category
Chemical Sciences Botany
Identifiers
URN: urn:nbn:se:umu:diva-123557DOI: 10.1111/pce.12719ISI: 000381496900012PubMedID: 26836813OAI: oai:DiVA.org:umu-123557DiVA: diva2:946807
Available from: 2016-07-06 Created: 2016-07-06 Last updated: 2016-09-13Bibliographically approved

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Shevela, DmitriyBerends, Hans-MartinKoroidov, SergeyMessinger, Johannes
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