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Replication-competent human adenovirus 11p vectors can propagate in Vero cells
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Virology.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Virology.
2016 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 495, 42-51 p.Article in journal (Refereed) PublishedText
Abstract [en]

The use of continuous cell lines derived from the African green monkey kidney (AGMK) has led to major advances in virus vaccine development. However, to date, these cells have not been used to facilitate the creation of human adenoviruses because most human adenoviruses undergo abortive infections in them. Here, we report the susceptibility of AGMK-derived cells to adenovirus lip (Ad11p) infection. First, we showed that CD46 molecules, which act as receptors for Ad11p, are expressed in AGMK cells. We then monitored Ad11p replication by measuring GFP expression as an indicator of viral transcription. We found that AGMK-derived cells were as capable as carcinoma cells at propagating full-length replication competent Ad11p (RCAd11p) DNA. Of the AGMK cell lines tested, Vero cells had the greatest capacity for adenovirus production. Thus, AGMK cells can be used to evaluate RCAd11p-mediated gene delivery, and Vero cells can be used for the production of RCAd11pGFP vectors at relatively high yields.

Place, publisher, year, edition, pages
2016. Vol. 495, 42-51 p.
Keyword [en]
Propagate, RCAd11pGFP vectors, Vero cells, LSERT C, 1984, VIRUS RESEARCH, V1, P365, FER C, 1990, JOURNAL OF VIROLOGY, V64, P3661
National Category
Immunology
Identifiers
URN: urn:nbn:se:umu:diva-124213DOI: 10.1016/j.virol.2016.04.029ISI: 000378659900005PubMedID: 27176913OAI: oai:DiVA.org:umu-124213DiVA: diva2:950675
Available from: 2016-08-01 Created: 2016-07-28 Last updated: 2016-08-01Bibliographically approved

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Mei, Ya-Fang
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