Change search
ReferencesLink to record
Permanent link

Direct link
Cytotoxic immune responses in the lungs correlate to disease severity in patients with hantavirus infection
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Infectious Diseases.
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Medicine.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Virology.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Immunology/Immunchemistry.
Show others and affiliations
2016 (English)In: European Journal of Clinical Microbiology and Infectious Diseases, ISSN 0934-9723, E-ISSN 1435-4373, Vol. 35, no 4, 713-721 p.Article in journal (Refereed) PublishedText
Abstract [en]

Hantavirus infections may cause severe and sometime life-threatening lung failure. The pathogenesis is not fully known and there is an urgent need for effective treatment. We aimed to investigate the association between pulmonary viral load and immune responses, and their relation to disease severity. Bronchoscopy with sampling of bronchoalveolar lavage (BAL) fluid was performed in 17 patients with acute Puumala hantavirus infection and 16 healthy volunteers acting as controls. Lymphocyte subsets, granzyme concentrations, and viral load were determined by flow cytometry, enzyme-linked immunosorbent assay (ELISA), and quantitative reverse transcription polymerase chain reaction (RT-PCR), respectively. Analyses of BAL fluid revealed significantly higher numbers of activated CD8(+) T cells and natural killer (NK) cells, as well as higher concentrations of the cytotoxins granzymes A and B in hantavirus-infected patients, compared to controls. In patients, Puumala hantavirus RNA was detected in 88 % of BAL cell samples and correlated inversely to the T cell response. The magnitude of the pulmonary cytotoxic lymphocyte response correlated to the severity of disease and systemic organ dysfunction, in terms of need for supplemental oxygen treatment, hypotension, and laboratory data indicating renal failure, cardiac dysfunction, vascular leakage, and cell damage. Regulatory T cell numbers were significantly lower in patients compared to controls, and may reflect inadequate immune regulation during hantavirus infection. Hantavirus infection elicits a pronounced cytotoxic lymphocyte response in the lungs. The magnitude of the immune response was associated with disease severity. These results give insights into the pathogenesis and possibilities for new treatments.

Place, publisher, year, edition, pages
2016. Vol. 35, no 4, 713-721 p.
National Category
Infectious Medicine Microbiology in the medical area
URN: urn:nbn:se:umu:diva-120634DOI: 10.1007/s10096-016-2592-1ISI: 000373300000023PubMedID: 26873376OAI: diva2:953269
Available from: 2016-08-17 Created: 2016-05-18 Last updated: 2016-08-17Bibliographically approved

Open Access in DiVA

fulltext(874 kB)15 downloads
File information
File name FULLTEXT01.pdfFile size 874 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Rasmuson, JohanPourazar, JamshidMohamed, NahlaLejon, KristinaEvander, MagnusBlomberg, AndersAhlm, Clas
By organisation
Infectious DiseasesMedicineVirologyImmunology/Immunchemistry
In the same journal
European Journal of Clinical Microbiology and Infectious Diseases
Infectious MedicineMicrobiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar
Total: 15 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 19 hits
ReferencesLink to record
Permanent link

Direct link