umu.sePublications
Change search
ReferencesLink to record
Permanent link

Direct link
Mitochondrial and Chloroplast Stress Responses Are Modulated in Distinct Touch and Chemical Inhibition Phases
Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
Show others and affiliations
2016 (English)In: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 171, no 3, 2150-2165 p.Article in journal (Refereed) Published
Abstract [en]

Previous studies have identified a range of transcription factors that modulate retrograde regulation of mitochondrial and chloroplast functions in Arabidopsis (Arabidopsis thaliana). However, the relative importance of these regulators and whether they act downstream of separate or overlapping signaling cascades is still unclear. Here, we demonstrate that multiple stress-related signaling pathways, with distinct kinetic signatures, converge on overlapping gene sets involved in energy organelle function. The transcription factor ANAC017 is almost solely responsible for transcript induction of marker genes around 3 to 6 h after chemical inhibition of organelle function and is a key regulator of mitochondrial and specific types of chloroplast retrograde signaling. However, an independent and highly transient gene expression phase, initiated within 10 to 30 min after treatment, also targets energy organelle functions, and is related to touch and wounding responses. Metabolite analysis demonstrates that this early response is concurrent with rapid changes in tricarboxylic acid cycle intermediates and large changes in transcript abundance of genes encoding mitochondrial dicarboxylate carrier proteins. It was further demonstrated that transcription factors AtWRKY15 and AtWRKY40 have repressive regulatory roles in this touch-responsive gene expression. Together, our results show that several regulatory systems can independently affect energy organelle function in response to stress, providing different means to exert operational control.

Place, publisher, year, edition, pages
American Society of Plant Biologists , 2016. Vol. 171, no 3, 2150-2165 p.
National Category
Genetics Botany
Identifiers
URN: urn:nbn:se:umu:diva-125557DOI: 10.1104/pp.16.00273ISI: 000381303300051PubMedID: 27208304OAI: oai:DiVA.org:umu-125557DiVA: diva2:970559
Available from: 2016-09-14 Created: 2016-09-13 Last updated: 2016-09-14Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Law, Simon R
By organisation
Umeå Plant Science Centre (UPSC)
In the same journal
Plant Physiology
GeneticsBotany

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

ReferencesLink to record
Permanent link

Direct link