umu.sePublications
Change search
ReferencesLink to record
Permanent link

Direct link
Chemometric Analysis of Bacterial Peptidoglycan Reveals Atypical Modifications That Empower the Cell Wall against Predatory Enzymes and Fly Innate Immunity
Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS).
Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS).
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Show others and affiliations
2016 (English)In: Journal of the American Chemical Society, ISSN 0002-7863, E-ISSN 1520-5126, Vol. 138, no 29, 9193-9204 p.Article in journal (Refereed) Published
Abstract [en]

Peptidoglycan is a fundamental structure for most bacteria. It contributes to the cell morphology and provides cell wall integrity against environmental insults. While several studies have reported a significant degree of variability in the chemical composition and organization of peptidoglycan in the domain Bacteria, the real diversity of this polymer is far from fully explored. This work exploits rapid ultraperformance liquid chromatography and multivariate data analysis to uncover peptidoglycan chemical diversity in the Class Alphaproteobacteria, a group of Gram negative bacteria that are highly heterogeneous in terms of metabolism, morphology and life-styles. Indeed, chemometric analyses revealed novel peptidoglycan structures conserved in Acetobacteria: amidation at the alpha-(L)-carboxyl of meso-diaminopimelic acid and the presence of muropeptides cross-linked by (1-3) L-Ala-D-(meso)diaminopimelate cross-links. Both structures are growth-controlled modifications that influence sensitivity to Type VI secretion system peptidoglycan endopeptidases and recognition by the Drosophila innate immune system, suggesting relevant roles in the environmental adaptability of these bacteria. Collectively our findings demonstrate the discriminative power of chemometric tools on large cell wall-chromatographic data sets to discover novel peptidoglycan structural properties in bacteria.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2016. Vol. 138, no 29, 9193-9204 p.
National Category
Chemical Sciences Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-125551DOI: 10.1021/jacs.6b04430ISI: 000380730000039PubMedID: 27337563OAI: oai:DiVA.org:umu-125551DiVA: diva2:971414
Available from: 2016-09-16 Created: 2016-09-13 Last updated: 2016-09-16Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Espaillat, AkbarForsmo, OskarBjörk, RafaelTrygg, JohanCava, Felipe
By organisation
Molecular Infection Medicine Sweden (MIMS)Department of Chemistry
In the same journal
Journal of the American Chemical Society
Chemical SciencesMicrobiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 42 hits
ReferencesLink to record
Permanent link

Direct link