Characterization of the plastid-localized inactive FtsHis of Arabidopsis thaliana
(English)Manuscript (preprint) (Other academic)
In the genome of Arabidopsis thaliana five genes encode members of the FtsH (Filamentation temperature sensitive protein H) protease family with mutations or deletions in their proteolytic site. Despite of not being active proteases these so called FtsHi (i for inactive) enzymes still seem to be highly important for plant development. All five enzymes have been localized within the plant chloroplast, most of them seem to be inserted in the chloroplast envelope. As homozygous ftshi mutants are seed lethal, here we compared different heterozygous ftshi mutants with respect to their growth at various light periods and at semi-natural growth conditions in the field. Their photosynthetic efficiency was evaluated by pulse-amplitude modulated fluorescence and the composition of their photosynthetic complexes was analysed by native polyacrylamide gel electrophoresis. Co-expression analyses were performed to find clues about the function of the five FtsHi inactive proteases.
Arabidopsis, chloroplast, inactive FtsH protease
Biochemistry and Molecular Biology Plant Biotechnology
Research subject Biochemistry
IdentifiersURN: urn:nbn:se:umu:diva-125815OAI: oai:DiVA.org:umu-125815DiVA: diva2:972476
FunderSwedish Energy Agency, 2012-005889