umu.sePublikasjoner
Endre søk
Begrens søket
1234567 1 - 50 of 547
RefereraExporteraLink til resultatlisten
Permanent link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Treff pr side
  • 5
  • 10
  • 20
  • 50
  • 100
  • 250
Sortering
  • Standard (Relevans)
  • Forfatter A-Ø
  • Forfatter Ø-A
  • Tittel A-Ø
  • Tittel Ø-A
  • Type publikasjon A-Ø
  • Type publikasjon Ø-A
  • Eldste først
  • Nyeste først
  • Skapad (Eldste først)
  • Skapad (Nyeste først)
  • Senast uppdaterad (Eldste først)
  • Senast uppdaterad (Nyeste først)
  • Disputationsdatum (tidligste først)
  • Disputationsdatum (siste først)
  • Standard (Relevans)
  • Forfatter A-Ø
  • Forfatter Ø-A
  • Tittel A-Ø
  • Tittel Ø-A
  • Type publikasjon A-Ø
  • Type publikasjon Ø-A
  • Eldste først
  • Nyeste først
  • Skapad (Eldste først)
  • Skapad (Nyeste først)
  • Senast uppdaterad (Eldste først)
  • Senast uppdaterad (Nyeste først)
  • Disputationsdatum (tidligste først)
  • Disputationsdatum (siste først)
Merk
Maxantalet träffar du kan exportera från sökgränssnittet är 250. Vid större uttag använd dig av utsökningar.
  • 1. Ahlgren, Camilla
    et al.
    Molin, Margareta
    Umeå universitet, Medicinsk fakultet, Odontologi, Protetik. Umeå universitet, Medicinsk fakultet, Odontologi.
    Lundh, Thomas
    Nilner, Krister
    Levels of gold in plasma after dental gold inlay insertion.2007Inngår i: Acta odontologica Scandinavica, ISSN 1502-3850, Vol. 65, nr 6, s. 331-4Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    OBJECTIVE: Several studies have reported increased levels of gold (Au) in the blood of patients with dental gold restorations. This study analyzed gold levels in blood plasma before dental gold inlay insertion, 0-12 months after, and 15 years after. MATERIAL AND METHODS: Plasma samples from 9 patients were taken before and 0-10 months after gold inlay insertion. Fifteen years after gold inlay insertion, further blood samples taken from 8 of these patients were analyzed for gold using inductively coupled plasma mass spectrometry. An oral examination was also carried out before and 15 years after gold inlay insertion. RESULTS: Gold levels in plasma were significantly higher 0-12 months after gold inlay insertion than before treatment (p=0.008). No significant difference in gold plasma levels was found between 0-12 months after and 15 years after insertion (p=0.109), although there was a significant correlation between the number of gold alloy surfaces and the amount of gold in plasma 15 years after insertion (p=0.028). CONCLUSIONS: This study supports a dose-related release of gold into plasma from dental gold restorations, a release that appears to be stable over time.

  • 2.
    Ahlqvist, Jan B
    et al.
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Nilsson, Tore A
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Hedman, Leif R
    Umeå universitet, Samhällsvetenskapliga fakulteten, Institutionen för psykologi.
    Desser, Terry S
    Dev, Parvati
    Johansson, Magnus
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Youngblood, Patricia L
    Cheng, Robert P
    Gold, Garry E
    A randomized controlled trial on 2 simulation-based training methods in radiology: effects on radiologic technology student skill in assessing image quality.2013Inngår i: Simulation in Healthcare: The Journal of the Society for Simulation in Healthcare, ISSN 1559-2332, E-ISSN 1559-713X, Vol. 8, nr 6, s. 382-387Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    INTRODUCTION: A simulator for virtual radiographic examinations was developed. In the virtual environment, the user can perform and analyze radiographic examinations of patient models without the use of ionizing radiation. We investigated if this simulation technique could improve education of radiology technology students. We compared student performance in the assessment of radiographic image quality after training with a conventional manikin or with the virtual radiography simulator.

    METHODS: A randomized controlled experimental study involving 31 first-year radiology technology students was performed. It was organized in 4 phases as follows: (I) randomization to control or experimental group based on the results of an anatomy examination; (II) proficiency testing before training; (III) intervention (control group, exposure and analysis of radiographic images of the cervical spine of a manikin; experimental group, exposure and analysis of the cervical spine images in the virtual radiography simulator); and (IV) proficiency testing after training.

    RESULTS: The experimental group showed significantly higher scores after training compared with those before training (P < 0.01). A linear mixed-effect analysis revealed a significant difference between the control and experimental groups regarding proficiency change (P = 0.01).

    CONCLUSIONS: Virtual radiographic simulation is an effective tool for learning image quality assessment. Simulation can therefore be a valuable adjunct to traditional educational methods and reduce exposure to x-rays and tutoring time.

  • 3.
    Ahlqvist, Jan, Birger
    et al.
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Borg, Kennet
    Gunne, Johan
    Nilson, Hans
    Olsson, Morgan
    Åstrand, Per
    Osseointegrated implants in edentulous jaws: a 2-year longitudinal study.1990Inngår i: International Journal of Oral & Maxillofacial Implants, ISSN 0882-2786, E-ISSN 1942-4434, Vol. 5, nr 2, s. 155-163Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Osseointegrated implants in 50 edentulous jaws were studied during a 2-year observation period. The implant survival rate was 89% in the maxillae and 97% in the mandibles. The marginal bone loss averaged 1.7 mm in the maxillae and 1.1 mm in the mandibles. Most of this bone loss occurred during the first year. The bone loss was greater in jaws with a preoperatively minor resorption of the alveolar ridge than in those with moderate or advanced resorption. The bone loss was also greater at the medially positioned implants than at those more posterior.

  • 4. Ahlqvist, Jan
    et al.
    Eliasson, S
    Welander, U
    The effect of projection errors on cephalometric length measurements1986Inngår i: European Journal of Orthodontics, ISSN 0141-5387, E-ISSN 1460-2210, Vol. 8, nr 3, s. 141-148Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The magnitude of projection errors, calculated theoretically on length measurements in cephalometry was studied. Rotation of the object by up to 5° from the proper position, resulted in errors in length measurements that were usually less than one percent. Rotations of more than 5° may increase the error but imply misalignments of the head that should be evident when positioning the patient. The advantage of extremely long focus film distances seems doubtful.

  • 5.
    Aili, Margareta
    Umeå universitet, Medicinska fakulteten, Molekylärbiologi (Teknat- och Medfak).
    Role of YopE and LcrH in effector translocation, HeLa cell cytotoxicity and virulence2005Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    In order to establish an extra-cellular infection the gram-negative bacteria Yersinia pseudotuberculosis uses a type III secretion system (T3SS) to translocate a set of anti-host effectors into eukaryotic cells. The toxins disrupt signalling pathways important for phagocytosis, cytokine production and cell survival. Secretion and translocation via this T3SS is strictly regulated on several levels. In this context, the function of YopE and LcrH during Yersinia infections has been analysed.

    YopE is an essential translocated effector that disrupts the actin cytoskeleton of infected eukaryotic cells, by inactivating small GTPases through its GTPase activating protein (GAP) activity. However, cytotoxicity can be uncoupled from in vitro GAP activity towards the RhoA, Rac1 and Cdc42 GTPases. Furthermore, in vivo studies of the YopE GAP activity revealed that only RhoA and Rac1 are targeted, but this is not a pre-requisite for Yersinia virulence. Hence, YopE must target one or more additional GTPases to cause disease in mice.

    YopE was the only Yersinia effector that blocks LDH release from infected cells. Moreover, translocated YopE could regulate the level of subsequent effector translocation by a mechanism that involved the YopE GAP function and another T3S component, YopK. Loss of translocation control elevated total T3S gene expression in the presence of eukaryotic cells. This indicated the existence of a regulatory loop for feedback control of T3S gene expression in the bacteria that originates from the interior of the eukaryotic cell after effector translocation is completed. This might represent the true virulence function of YopE.

    Exoenzyme S (ExoS) of Pseudomonas aeruginosa has a YopE-like GAP domain with similar activity towards RhoA, Rac1 and Cdc42. However, ExoS is unable to complement hyper-translocation resulting from loss of YopE. This indicates a unique function for YopE in translocation control in Yersinia that might be dependent on correct intracellular localisation. It follows that the Membrane Localisation Domain in YopE was important for translocation control, but dispensable for cytotoxicity and blockage of LDH release.

    YopD and its cognate chaperone LcrH are negative regulatory elements of the T3S regulon and together with YopB, are involved in the effector translocation process. Randomly generated point mutants in LcrH specifically effected stability and secretion of both the YopB and YopD substrates in vitro and prevented their apparent insertion as translocon pores in the membranes of infected cells. Yet, these mutants still produced stable substrates in the presence of eukaryotic cells and most could mediate at least partial effector translocation. Thus, only minimal amounts of the YopB and YopD translocator proteins are needed for translocation and the LcrH chaperone may regulate this process from inside the bacteria.

  • 6.
    Albertsson, Katarina Wikén
    et al.
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    van Dijken, Jan W V
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Awareness of toothbrushing and dentifrice habits in regularly dental care receiving adults2010Inngår i: Swedish Dental Journal, ISSN 0347-9994, Vol. 34, nr 2, s. 71-78Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The aim of this study was to investigate toothbrushing and dentifrice habits in a Swedish adult population with relatively high caries frequency, which received regularly dental care and to evaluate the awareness of their toothbrush technique. Sixty adult participants with high caries frequency, 29 woman and 31 men, answered a self-reporting questionnaire with 42 questions concerning their oral care, brushing technique and -habits.The responses were related to their clinical behaviour observed during a customary toothbrushing session. Fifty-three participants fulfilled both the questionnaire and the clinical observation. Half of these used toothpaste containing 1450-1500 ppm fluoride but only one of all participants was aware of the fluoride concentration used. The majority used a manual toothbrush and 95% brushed their teeth twice a day using 0.9 g toothpaste. A wide range of brushing methods and habits was observed. Sixty percent did not brush systematically. Spitting of toothpaste-saliva during brushing was performed by 60% and after brushing by 15%.The observed brushing times were significantly higher than the self reported. The observed brushing times were <1 min: 3.4%, 1-2 min: 36.7% and >2 min: 47.0%. There was a significant correlation between observed brushing time and caries activity. Rinsing with water after brushing was performed once (32%) or twice (44%) during the observations. Only 9% rinsed with toothpaste slurry after brushing. It can be concluded that the awareness of the individual toothbrushing, post-brushing behaviour and the use of fluoride toothpaste was non-optimal in the adult participants. Oral health promotion by optimalized use of fluoride toothpaste and improved post-brushing behaviour should be recommended.

  • 7. Alghadir, Ahmad
    et al.
    Aly, Farag
    Zafar, Hamayun
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi, Klinisk oral fysiologi.
    Effect of face veil on ventilatory function among Saudi adult females2012Inngår i: Pakistan journal of medical sciences print, ISSN 1682-024X, E-ISSN 1681-715X, Vol. 28, nr 1, s. 71-74Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Objective: The use of face veil called "niqab" by women to cover their faces at public places is a common practice in some Muslim communities. The long-term effect of niqab use on ventilatory function (VF) has not previously been reported. The aim of this cross-sectional study was to compare VF between niqab wearing and non-niqab wearing healthy Saudi females. Methodology: Thirty eight healthy adult Saudi females participated in this study. Nineteen subjects were regular niqab users and the other nineteen were either not using niqab at all or used it for less than one hour per day. Forced vital capacity (FVC), forced expiratory volume in one second (FEV1), FEV1/FVC (%), and maximal voluntary ventilation (MVV) were recorded using a digital spirometer. Results: Mean values of FVC, FEV1, FEV1/FVC (%) and MVV for niqab wearers were significantly lower than the corresponding values for non-niqab wearers. Significant negative correlation was found between the FVC and FEV1 values and the number of hours of the use of face veil per day. Conclusions: Long-term use of traditional niqab use can affect VF.

  • 8. Alghadir, Ahmad H.
    et al.
    Zafar, Hamayun
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Iqbal, Zaheen A.
    Effect of three different jaw positions on postural stability during standing2015Inngår i: Functional Neurology, ISSN 0393-5264, E-ISSN 1971-3274, Vol. 30, nr 1, s. 53-57Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Studies in the literature show that jaw and neck regions are linked anatomically, biomechanically and neurologically. Voluntary clenching has been shown to improve muscle strength and performance of various motor tasks. Information from the neck sensory-motor system is reported to be important for posture. Hence it is reasonable to believe that activation of the jaw sensory-motor system has the potential to modulate posture. In a sample of 116 healthy subjects, we compared center of gravity (COG) velocity during quiet standing on a foam surface during three test positions: i) resting jaw, ii) open jaw, and iii) clenching; these were tested in two conditions: with eyes open and with eyes closed. The COG velocity decreased significantly during clenching in comparison to both open and resting jaw positions (p<0.0001). This suggests that the jaw sensory-motor system can modulate postural mechanisms. We conclude that jaw clenching can enhance postural stability during standing on an unstable surface in both the presence and absence of visual input in healthy adults and suggest that this should be taken into consideration in treatment and rehabilitation planning for patients with postural instability.

  • 9. Alghadir, Ahmad H.
    et al.
    Zafar, Hamayun
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi. Department of Rehabilitation Sciences, College of Applied Medical Sciences, King Saud University, Riyadh.
    Iqbal, Zaheen A.
    Effect of tongue position on postural stability during quiet standing in healthy young males2015Inngår i: Somatosensory & motor research, ISSN 0899-0220, E-ISSN 1369-1651, Vol. 32, nr 3, s. 183-186Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background and aims: Role of the neck and jaw sensory motor system in control of body balance has been established. Tongue is an integral part of jaw sensory motor system and helps in execution of purposeful and precise motor tasks like eating, drinking and speaking. The purpose of this study was to evaluate the possible effects of tongue position on the postural control system. Materials and method: We compared the mean center of gravity (COG) velocity during quiet standing on an unstable surface with eyes closed during two test conditions: (i) with habitual jaw resting position and (ii) with instructed tongue positioned against the upper incisors. One hundred and sixteen normal healthy male subjects (average age 31.56 +/- 8.51 years and height 170.86 +/- 7.26 cm) participated in the study. Their COG velocity (deg/s) was measured using the NeuroCom (R) Balance Master version 8.5.0 (Clackamas, OR, USA). Results and conclusions: The results show that COG velocity decreased significantly while tongue was positioned against upper incisors in comparison to the habitual jaw resting position. Our findings suggest that the tongue positioning can modulate postural control mechanisms. Tongue positioning against the upper incisors can enhance the postural stability during upright standing on an unstable surface and in the absence of vision in healthy young adults. Our findings can be of value for evaluation and rehabilitation protocols for postural control dysfunction.

  • 10. Alghadir, Ahmad
    et al.
    Zafar, Hamayun
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi. King Saud Univ, Coll Appl Med Sci, Rehabil Res Chair, Riyadh, Saudi Arabia.
    Iqbal, Zaheen A.
    Al-Eisa, Einas
    Work-Related Low Back Pain Among Physical Therapists in Riyadh, Saudi Arabia2017Inngår i: Workplace Health & Safety, ISSN 2165-0799, Vol. 65, nr 8, s. 337-345Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Low back pain (LBP) is a common health problem. Professions like physical therapy (PT), involving frequent lifting, bending, or standing, are at risk for developing LBP. The objective of this study was to determine the prevalence of work-related LBP and factors associated with and consequences of work-related LBP among physical therapists in Riyadh, Saudi Arabia. A self-administered online questionnaire (i.e., demographic data, history of LBP before and after working as a physical therapist, work setting, and effect on daily activities) was sent to 600 members of the Saudi PT association. Data were analyzed using the Pearson chi-square test and Mann-Whitney U test. Eighty-eight percent of potential respondents completed the questionnaire. Of these, 89.65% of the therapists reported LBP after beginning their PT practice, and 35.6% reported LBP at the time of this survey. Gender, PT specialty, and duration of contact with patients were all found to be related to LBP. The prevalence of work-related LBP among physical therapist in Riyadh was high, affecting patient care and daily activities of the therapists. Both primary and secondary prevention strategies (e.g., introduce ergonomics into PT curricula, reduce therapist stress, and promote teamwork) are needed to decrease LBP among therapists, so they can effectively care for patients.

  • 11. Alghadir, Ahmad
    et al.
    Zafar, Hamayun
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi. Department of Rehabilitation Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.
    Whitney, S. L.
    Iqbal, Zaheen
    Effect of chewing on postural stability during quiet standing in healthy young males2015Inngår i: Somatosensory & motor research, ISSN 0899-0220, E-ISSN 1369-1651, Vol. 32, nr 2, s. 72-76Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background and aims: There is an important role of the neck sensory motor system in control of body posture and balance, and it is reasonable to believe that the jaw sensory motor system can directly and indirectly influence the modulation of the postural control system. The purpose of this study was to evaluate possible effects of dynamic jaw position while chewing on the postural control system. Materials and methods: We compared the mean center of gravity (COG) velocity during quite standing on a foam surface with eyes closed during three test conditions: (i) with resting jaw position, (ii) with open jaw position, and (iii) while chewing standard bolus of chewing gum. One hundred and sixteen normal healthy male subjects (average age 31.56 +/- 8.51 years; height 170.86 +/- 7.26 cm) were recruited for the study. Their COG velocity (deg/s) was measured using the NeuroCom (R) Balance Master Version 8.5.0 (Clackamas, OR, USA). Statistical analysis: Data was tested by the Friedman test. Results and conclusions. The results show that COG velocity decreased significantly while chewing in comparison to both open and resting jaw position (p < 0.0001). Our finding corroborates previous studies and suggests that the jaw sensory motor system can modulate postural control mechanisms. Gum chewing activity can enhance the postural stability during upright standing on an unstable surface and in the absence of visual input in healthy young adults. Our results should be taken into consideration in treatment and rehabilitation planning for patients with postural instability.

  • 12. Almosa, Naif
    et al.
    Zafar, Hamayun
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Incidence of orthodontic brackets detachment during orthodontic treatment: A systematic review2018Inngår i: Pakistan journal of medical sciences print, ISSN 1682-024X, E-ISSN 1681-715X, Vol. 34, nr 3, s. 744-750Artikkel, forskningsoversikt (Fagfellevurdert)
    Abstract [en]

    Objectives: To evaluate the incidence of orthodontic brackets detachment during orthodontic treatment.

    Methods: Using electronic databases; eligible studies up to January 2018 were retrieved, independently reviewed, and screened. The Coleman Methodology Scoring System (CMS) and Cochrane Collaboration's tool were used to assess quality and risk of bias in the included studies.

    Results: Of the seventeen studies included in the final synthesis, thirteen were categorized as randomized clinical trials (RCTs), one prospective cohort and retrospective survey each, whereas two studies could not be categorized. The number of patients in the selected studies ranged between 19 and 153; the mean age was between 10.5 to 38.7 years, and male to female ratio was 353:495. Almost all studies had a high risk of bias, and more than half of the studies had CMS score of 70 or above. The numbers of brackets examined in the studies ranged between 361 and 3336. The incidence of brackets detachment ranged from 0.6 to 28.3%.

    Conclusions: The incidence of brackets detachment during orthodontic treatment is high.

  • 13.
    Al-Taai, Nameer
    et al.
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Alfatlawi, Fakhri
    Univ Baghdad, Coll Dent, Dept Orthodont, Baghdad, Iraq.
    Ransjö, Maria
    Univ Gothenburg, Sahlgrenska Acad, Dept Orthodont, Gothenburg, Sweden.
    Fakhry, Saad
    Univ Al Nahrain, Coll Med, Dept Endocrinol & Metab, Baghdad, Iraq.
    Effect of rapid maxillary expansion on monosymptomatic primary nocturnal enuresis2015Inngår i: Angle orthodontist, ISSN 0003-3219, E-ISSN 1945-7103, Vol. 85, nr 1, s. 102-108Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Objective: To evaluate the effects of rapid maxillary expansion (RME) on nocturnal enuresis (NE) related to the nasal airway, nasal breathing, and plasma osmolality (as an indicator for antidiuretic hormone). Materials and Methods: Nineteen patients with monosymptomatic primary NE, aged 6-15 years, were treated with RME for 10-15 days. To exclude a placebo effect of the RME appliance, seven patients were first treated with a passive appliance. Computed tomography of nasal cavity, rhinomanometric, and plasma osmolality measurements were made 2-3 days before and 2-3 months after the RME period. RME effects on NE were followed for three more years. Results: Two to three months after the expansion there were significant improvements in the breathing function and a decrease in the plasma osmolality. NE decreased significantly in all patients after the RME period, and all patients showed full dryness after 3 years. Conclusions: This study demonstrates that RME causes complete dryness in all patients, with significant effects on pathophysiological mechanisms related to NE.

  • 14. Andersson, A
    et al.
    Sköld-Larsson, K
    Hallgren, A
    Pettersson, LG
    Twetman, Svante
    Umeå universitet, Medicinsk fakultet, Odontologi.
    White spot lesion regression with the CPP-ACP system assessed by a laser fluorescence device (DIAGNOdent).2006Inngår i: Oral Health Prev DentArtikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Purpose: To investigate and compare the effects of a dental cream containing complexes of casein phosphoprotein-amorphous calcium phosphate (CPP-ACP) and fluoride mouth rinses on the regression of white spot lesions (WSL). Material and methods: The study group consisted of 26 healthy adolescents (mean age 14.6 yr) exhibiting 60 teeth with 152 visible WSL sites on incisors and canines immediately after debonding of fixed orthodontic appliances. After bracket removal, professional tooth cleaning and drying, a visual scoring (0-4) and laser fluorescence (LF) readings were carried out. The patients were randomly assigned to two different treatment protocols with the aim to remineralize the lesions: A) daily topical applications of a dental cream containing CPP-ACP (Topacal) for 3 months followed by a 3-month period of daily tooth brushing with fluoridated toothpaste, or B) daily 0.05% sodium fluoride rinses combined with fluoridated toothpaste for 6 months. The registrations were repeated after 1, 3, 6 and 12 months and follow-up data were compared with baseline with aid of chi-square and paired t-tests. Results: A significant improvement of the clinical WSL-scores was found over time in both groups but there was a statistically significant difference (p<0.01) concerning the number of sites that totally disappeared after 12 months in favor for the CPP-ACP regime, 63% vs. 25% respectively. The clinical registrations were mirrored by a statistically significant decrease (p<0.05) in the LF readings at the 6- and 12-month follow-ups compared to baseline. No significant differences were displayed between the groups. Conclusion: Clinical scoring and LF assessment suggested that both regimens could promote regression of WSL after debonding of fixed orthodontic appliances. The visual evaluation suggested an aesthetically more favourable outcome of the amorphous calcium phosphate treatments.

  • 15.
    André, Maria
    et al.
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Kou, Wen
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Sjögren, Göran
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Sundh, Anders
    Research and Development, Cad.esthetics AB, Skellefteå, Sweden.
    Effects of pretreatments and hydrothermal aging on biaxial flexural strength of lithium di-silicate and Mg-PSZ ceramics2016Inngår i: Journal of Dentistry, ISSN 0300-5712, E-ISSN 1879-176X, Vol. 55, s. 25-32Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Objectives: To evaluate the effect of specimen thickness, pretreatment and hydrothermal aging on the biaxial flexural strength (BFS) of lithium di-silicate glass (e.max Cad) and magnesia-stabilized zirconia (ZirMagnum) ceramic discs. Methods: The e.max Cad discs was studied: i) crystallized, ii) crystallized and glazed and iii) crystallized, glazed and unglazed side etched with hydrofluoric acid. The ZirMagnum discs were studied: i) as delivered, ii) after sandblasting and iii) after heat treatment similar to veneering. Hydrothermal aging was simulated by autoclave treatment. Results: The BFS of all the ZirMagnum specimens was superior (p < 0.001) to all the e.max Cad specimens. Glazing the 0.4 mm e.max Cad discs reduced (p < 0.05) their BFS compared with the unglazed 0.8 mm specimens, whereas glazing of 0.8 mm discs had no influence (p > 0.05) on the strength. Etching and autoclaving of e.max Cad did not affect (p > 0.05) the BFS. For ZirMagnum sandblasting with 0.2 MPa or 0.6 MPa did not influence the biaxial flexural strength (p > 0.05), whereas heat treatment reduced (p < 0.01) the BFS of 0.6 MPa sandblasted ZirMagnum. Autoclaving reduced the strength (p < 0.05) compared with ZirMagnum as delivered, whereas autoclaving of the 0.6 MPa sandblasted and heat treated specimens did not influence (p > 0.05) the BFS. Glazing, etching and sandblasting increased (p < 0.05) surface roughness. Conclusions: The effects of glazing, heat treatment, aging and mechanical treatment of the materials evaluated should be considered since their strength could be affected. Clinical significance: Mechanical properties of restorations made from prefabricated ceramic blocks could be affected of various treatments and could change over time.

  • 16.
    Asikainen, Sirkka E
    Umeå universitet, Medicinsk fakultet, Odontologi, Oral mikrobiologi.
    Periodontal bacteria and cardiovascular problems.2009Inngår i: Future microbiology, ISSN 1746-0921, Vol. 4, s. 495-498Artikkel i tidsskrift (Annet vitenskapelig)
  • 17.
    Asikainen, Sirkka
    et al.
    Umeå universitet, Medicinsk fakultet, Odontologi.
    Karched, Maribasappa
    Umeå universitet, Medicinsk fakultet, Odontologi.
    Molecular techniques in oral microbial taxonomy, identification and typing2008Inngår i: Molecular Oral Microbiology, Caister Academic Press, Norfolk UK , 2008, s. 1-27Kapittel i bok, del av antologi (Fagfellevurdert)
    Abstract [en]

    The rapid development of molecular techniques during the past decade has revolutionized the field of microbiology. Two issues are of profound importance. First, the discovery of phylogenetically informative DNA sequences, such as the 16S rRNA gene, radically changed the concept of bacterial relatedness and provided a universal system for bacterial identification and categorization. Second, it became possible to detect, identify, and type bacteria independent of their cultivability and, by these new means, to elucidate the diversity and spatial organization of complex oral bacterial communities. Of considerable benefit has been the fact that the same nucleic acid-based molecular approaches can be applied in all microbial environments, ranging from the oral cavity, to the surfaces of historical monuments, to the depths of open oceans. This has led to development of versatile PCR- and hybridization-based techniques that allow a rapid and convenient analysis of the bacterial contents of oral samples and offer previously unattainable possibilities for expanding studies on bacterial epidemiology and characterization. In particular, the emerging new microarray technology will facilitate great strides in understanding the structure and dynamics of oral bacterial communities and bacteria-host interactions, and will form the basis for developing novel diagnostics for oral infections.

  • 18.
    Asplund, Kjell
    Umeå universitet, Medicinska fakulteten, Institutionen för folkhälsa och klinisk medicin, Medicin.
    Hur kan tandhälsans stora gap bestå?2017Inngår i: Tandläkartidningen, ISSN 0039-6982, nr 2, s. 37-37Artikkel i tidsskrift (Annet vitenskapelig)
  • 19. Astvaldsdottir, Alfheidur
    et al.
    Dagerhamn, Jessica
    van Dijken, Jan W. V.
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi, Tandhygienistutbildning.
    Naimi-Akbar, Aron
    Sandborgh-Englund, Gunilla
    Tranaeus, Sofia
    Nilsson, Mikael
    Longevity of posterior resin composite restorations in adults: A systematic review2015Inngår i: Journal of Dentistry, ISSN 0300-5712, E-ISSN 1879-176X, Vol. 43, nr 8, s. 934-954Artikkel, forskningsoversikt (Fagfellevurdert)
    Abstract [en]

    Objective: To conduct a systematic review of the literature on the longevity of posterior resin composite restorations in adults. Material and methods: A systematic literature search was conducted according to predetermined criteria for inclusion and exclusion. The studies selected were prospective clinical trials with a minimum follow-up time of 4 years, 40 restorations per experimental group and an annual attrition rate of less than 5%. Initially, abstracts and full-text articles were assessed independently and the assessment was subsequently agreed on by five reviewers. The methodological quality of the studies was assessed according to the Swedish Council on Health Technology Assessment (SBU) standard checklist for determining the extent to which studies meet basic quality criteria. Results: In all, the literature search identified 4275 abstracts and 93 articles were read in fulltext. There were eighteen studies which met the criteria for inclusion, eight of which were included in the analysis. There were 80 failures of restorations with a total follow-up time at risk for failure of 62,030 months. The overall incidence rate for all causes of failure was 1.55 lost restorations per 100 restoration years. The most common biological reason for failure (a total of 31 restorations) was secondary caries, with or without fracture of the restoration. The quality of the evidence was low. Conclusions: In an efficacy setting, the overall survival proportion of posterior resin composite restorations is high. The major reasons for failure are secondary caries and restoration fracture which supports the importance of adequate follow-up time. Clinical significance: The overall survival proportion of posterior composite restorations was high, but the results cannot be extrapolated to an effectiveness setting. The importance of adequate follow-up time is supported by the finding that secondary caries often occurred after 3 years or later.

  • 20. Axelsson, S
    et al.
    Söder, B
    Nordenram, G
    Pettersson, LG
    Dahlgren, H
    Norlund, A
    Källestål, C
    Mejare, I
    Lingström, P
    Holm, Anna-Karin
    Umeå universitet, Medicinsk fakultet, Odontologi, Pedodonti.
    Twetman, Svante
    Umeå universitet, Medicinsk fakultet, Odontologi, Pedodonti.
    Effect of combined caries-preventive methods: a systematic review of controlled clinical trials.2004Inngår i: Acta odontologica Scandinavica, Vol. 62, nr 3, s. 163-9Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The aim of this systematic review was to evaluate the caries-preventive effect of combined caries-preventive methods, defined as two or more different interventions in combination, each expected to prevent dental caries. The Medline database was searched for articles published in the period January 1966 to June 2003. Twenty-four controlled studies met the inclusion criteria, and their value as evidence was assessed according to predetermined criteria. The level of evidence for the overall conclusion regarding each method was graded according to the protocol of the Swedish Council on Technology Assessment in Health Care. The scientific evidence for the combination of treatments involving fluoride that had a preventive effect on caries in children and adolescents was graded as moderate. However, for elderly patients the scientific evidence for the caries-preventive effect of different combinations of treatments was found to be incomplete. No conclusion could be drawn regarding the evidence for combinations of treatments being effective for groups at high caries risk, as the results from the identified clinical studies were conflicting.

  • 21. Becker, William
    et al.
    Sennerby, Lars
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi, Käkkirurgi. Department of Biomaterials, Institute for Surgical Sciences, University of Gothenburg.
    Bedrossian, Edwin
    Becker, Burton E
    Lucchini, Jean Pierre
    Implant stability measurements for implants placed at the time of extraction: a cohort, prospective clinical trial2005Inngår i: Journal of Periodontology, ISSN 0022-3492, E-ISSN 1943-3670, Vol. 76, nr 3, s. 391-397Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background: Studies indicate that implants placed at the time of extraction have high success rates. Implants must be stable at the time of insertion. Presently there are no data indicating the degree of implant stability when implants are placed at the time of extraction. This study evaluated changes in stability of implants from implant placement to abutment connection utilizing resonance frequency analysis (RFA). The unit of measurement was the international stability quotient (ISQ).

    Methods: Prior to treatment, patients were given medical history and dental evaluations. Periapical and panogram radiographs were taken. Fifty-two patients requiring extraction of one or two teeth and implant placement immediately after extraction were enrolled in this study. Under conscious sedation and local anesthesia or local anesthesia alone, teeth were atraumatically removed and the extraction sockets were debrided. A total of 73 dental implants (57 in the maxilla, 16 in the mandible) were placed. Using a one-stage approach, all implants were placed within the patient's alveolar envelope and were never placed directly into extraction sockets. All implants were placed into contained extraction sites. Bone augmentation procedures were not performed. After implant insertion, the RFA electronic transducer was attached to the head of the implant with the retaining screw. The device was attached to a computer designed to register RFA scores in ISQ units. RFA measurements were taken at implant placement and abutment connection. Bone qualities, quantity, implant length and width as well as site of placement were recorded.

    Results: The average interval between implant insertion and abutment connection was 5.6 months (SD 2.05). Two implants were lost between implant insertion and 1 year. At 2 to 3 years, the cumulative survival was 97.2%. Resonance frequency measurements at implant placement showed a mean primary stability of 62.0 (SE 1.1; range 43 to 83 ISQ) and a mean secondary stability after 1 year of 64.0 (SE 1.2; range 40 to 98 ISQ) for all implants. The increase was marginally significant (generalized estimating equation z-statistic = 1.79; P value = 0.07).

    Conclusions: Implants placed at the time of extraction and inserted into native bone and not directly into extractions sockets have a high degree of initial stability as evidenced by RFA measurements. Implants with initial high ISQ levels revealed a slight drop in levels over time, while implants with levels lower than 60 had increases in levels between implant insertion and abutment connection. At 2 to 3 years the cumulative survival rate was 97.2%.

  • 22. Belibasakis, George
    et al.
    Brage, Monica
    Umeå universitet, Medicinsk fakultet, Odontologi, Parodontologi. Umeå universitet, Medicinsk fakultet, Medicinsk biovetenskap, Patologi.
    Lagergård, Teresa
    Johansson, Anders
    Umeå universitet, Medicinsk fakultet, Odontologi.
    Cytolethal distending toxin upregulates RANKL expression in Jurkat T-cells: Cdt upregulates RANKL2008Inngår i: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 116, nr 6, s. 499-506Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Cytolethal distending toxin, a bacterial exotoxin produced by a number of Gram-negative species, causes growth arrest and morphological alterations in host cells. Among these species are Haemophilus ducreyi, the etiological agent of chancroid, and the periodontal pathogen Aggregatibacter actinomycetemcomitans, highly implicated in localized aggressive periodontitis. CDT induces receptor activator of NF-kappaB ligand (RANKL) expression in periodontal fibroblasts, the key bone-resorbing cytokine. T-cells are actively involved in localized inflammation-induced bone destruction, including periodontitis. The aim of this study was to investigate the effects of purified CDT on the expression of RANKL and its decoy receptor osteoprotegerin (OPG), in the Jurkat T-cell line. Quantitative real-time PCR indicated that 100 pg/ml of purified H. ducreyi CDT upregulated RANKL mRNA expression by 2.2-fold, after 24 h of exposure. This increase was corroborated by a 2.0-fold increase in RANKL protein release, as determined by ELISA. OPG was not detected in this experimental system. In conclusion, CDT enhances RANKL expression in T-cells, denoting that these cells are a potential target for the toxin and strengthening the potential link between this virulence factor and mechanisms associated with localized bone resorption.

  • 23. Belibasakis, Georgios N
    et al.
    Johansson, Anders
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi, Molekylär paradontologi.
    Aggregatibacter actinomycetemcomitans targets NLRP3 and NLRP6 inflammasome expression in human mononuclear leukocytes2012Inngår i: Cytokine, ISSN 1043-4666, E-ISSN 1096-0023, Vol. 59, nr 1, s. 124-130Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Periodontitis is an inflammatory condition that destroys the tooth-supporting tissues, as a result of local bacterial infection. Aggregatibacter actinomycetemcomitans is a Gram-negative facultative anaerobic species, highly associated with aggressive periodontitis. Periodontal inflammation is dominated by cytokines of the Interleukin (IL)-1 family. Prior to their secretion by mononuclear cells, IL-1 cytokines are processed by intracellular protein complexes, known as "inflammasomes", which can sense the bacterial challenge. The aim of this study was to investigate which inflammasomes are regulated in mononuclear cells in response to A. actinomycetemcomitans. The D7SS strain and its derivative leukotoxin and cytolethal distending toxin knock-out mutant strains were used to infect human mononuclear cells at a 1:10 cell: bacteria ratio, for 3h. The expression of various inflammasome components in the cells was investigated by TaqMan quantitative real-time polymerase chain reaction (qPCR). The expressions of NOD-like receptor protein (NLRP)1, NLRP2 and Absent In Melanoma (AIM)2 inflammasome sensors, as well as their effector Caspase-1 were not affected. However, NLRP3 was up-regulated, while NLRP6 was down-regulated. This effect was not dependent on the leukotoxin or the cytolethal distending toxin, as demonstrated by the use of specific gene knock-out mutant strains. IL-1β and IL-18 expressions were also up-regulated by the bacterial challenge. In conclusion, A. actinomycetemcomitans enhances NLRP3 and reduces NLRP6 inflammasome expression, irrespective of its major virulence factors, confirming the high pathogenic profile of this species, and providing further insights to the mechanisms of periodontal inflammation.

  • 24. Benteke, M
    et al.
    Berntsson, L
    Broman, U
    Enfeldt, K
    Sköld-Larsson, K
    Twetman, S
    Umeå universitet, Medicinsk fakultet, Odontologi, Pedodonti.
    Population- vs. risk-based applications of fissure sealants in first permanent molars: a 13-year follow-up.2006Inngår i: Oral health & preventive dentistry, Vol. 4, nr 151-156Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    PURPOSE: To evaluate and compare the long-term outcome of a population-based and a risk-based strategy of applying fissure sealants in newly erupted first permanent molars. DESIGN AND SETTING: Retrospective cohort study in primary dental care. MATERIALS AND METHODS: Four hundred and ninety-six subjects from two cohorts were included. In group P (n = 236), routine applications of fissure sealants were conducted while sealants were placed on the basis of subjective criteria in Group R (n = 260). Data of applications, maintenance, decay, restorations, extractions, and cumulative time of applications from six to 19 years were extracted from the dental records and bite-wing radiographs by two of the authors simultaneously. The main outcome measures were the total caries prevalence at the age of 19 years as well as the fate of each sealed and non-sealed occlusal surface. RESULTS: In group P, 87.7% of all first permanent molars were sealed compared with 20.2% in group R. There was no statistically significant difference concerningthe total DFT at 19 years of age between the groups (mean 3.5 +/- 3.8 vs. 3.7 +/- 3.4). Considering the occlusal surfaces of the first permanent molars only, a statistically significant treatment effect was demonstrated in group P with an odds ratio of 4.6 (95% CI: 3.0 - 6.7; p < 0.05). The absolute risk reduction was 36.3% and the number needed to treat was 2.8. In Group R, no statistically significant treatment effect was disclosed (OR = 1.3; 95% CI: 0.9 - 1.7; risk reduction 5.5%). CONCLUSION: This 13-year follow-up showed that population-based applications of fissure sealants in first permanent molars shortly after eruption showed a statistically significant treatment effect on the occlusal surfaces at the age of 19 years, which was in contrast to findings from a cohort in which sealants were applied on the basis of an individual risk-based strategy. There were, however, no significant differences concerning the total caries experience between the two cohorts

  • 25.
    Bergdahl, Jan
    et al.
    Umeå universitet, Samhällsvetenskapliga fakulteten, Institutionen för psykologi.
    Mårell, Lena
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Bergdahl, Maud
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Perris, Hjördis
    Psychobiological personality dimensions in two environmental-illness patient groups2005Inngår i: Clinical Oral Investigations, ISSN 1432-6981, E-ISSN 1436-3771, Vol. 9, nr 4, s. 251-256Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    The aim of the present study was to investigate the psychobiological personality dimensions in two subgroups of patients with environmental illness (EI). Fifty-nine patients, 34 women and 25 men (aged 32-69 years), were referred for symptoms allegedly caused by abnormal sensitivity to either dental fillings (DF; n=26) or electromagnetic fields (EMF; n=33). For the evaluation of personality, the Swedish 238-item version of the Temperament and Character Inventory (TCI) was used. Compared with a control group, the EMF group scored higher on the temperament dimension Persistence. The DF group scored higher on the TCI subscales Harm Avoidance (fatigability and asthenia) and Self-Directedness (self-acceptance). Women scored higher than men did on the Novelty Seeking and Reward Dependence (RD) dimensions in the DF group and on RD in the control group, indicating an inherited gender difference. No differences were found between men and women in the EMF group. Our results indicate that the high level of persistence found in the EMF group and the high level of fatigability and asthenia in combination with high self-acceptance found in the DF group represent vulnerable personalities. No significant differences were found between the two patient groups, indicating that these groups are quite similar regarding personality. This vulnerability can be expressed as various mental and somatic symptoms, which can be interpreted as El symptoms by the affected individual.

  • 26.
    Bergdahl, M
    et al.
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Hedström, L
    Metronidazole for the prevention of dry socket after removal of partially impacted mandibular third molar: a randomised controlled trial2004Inngår i: British Journal of Oral & Maxillofacial Surgery, ISSN 0266-4356, E-ISSN 1532-1940, Vol. 42, nr 6, s. 555-558Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    We randomised 119 patients who had been referred for removal of partially impacted mandibular third molars to be given either metronidazole 1600 mg or placebo as a single dose 45 min before operation. Ten of the fifty-nine patients who were given metronidazole and 13 of the 60 given placebo developed dry sockets. Two variables were significantly associated with the development of a dry socket: pericoronitis and oral contraceptives.

  • 27.
    Bergendal, Birgitta
    Umeå universitet, Medicinsk fakultet, Odontologi.
    Oligodontia and ectodermal dysplasia: on signs, symptoms, genetics and outcomes of dental treatment2010Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    The general aim of this thesis was to broaden our knowledge of the signs and symptoms, genetics, and outcomes of dental implant treatment in individuals with oligodontia or ectodermal dysplasia.

    Article I is a population-based study in three Swedish counties of 162 individuals with oligodontia, which was a prevalence of 0.09%. The intent was to explore ways for dentists to assess symptoms from other ectodermal structures than teeth through a clinical interview and chair-side analyses. Thirty per cent had low salivary secretion rates while only 11% with no known syndrome reported symptoms from hair, nails, or sweat glands. These are, together with teeth, the ectodermal structures on which it is proposed that a clinical diagnosis of ectodermal dysplasia (ED) be based.

    Article II screened 93 probands with oligodontia for mutations in six genes known to cause oligodontia and hypohidrotic ED. Sequence alterations predicted to be damaging or potentially damaging were revealed in the AXIN2, MSX1, PAX9, and EDARADD genes in 14 (15%) of the probands. All mutations but one were novel. For the first time, EDARADD mutations were shown to cause isolated oligodontia. No individual who had reported ectodermal symptoms from hair, nails, or sweat glands had a mutation.

    Article III assessed orofacial function in individuals with different types of EDs using the Nordic Orofacial Test-Screening (NOT-S) protocol. Individuals with ED scored significantly higher in orofacial dysfunction than a healthy reference sample, especially in the Chewing and swallowing, Dryness of the mouth, and Speech domains.

    Article IV surveyed treatment outcome of dental implants in Swedish children up to age 16 years. In a 20-year period, only 26 patients were treated, 5 of whom had hypohidrotic ED and anodontia of the mandible. Individuals with ED had 64% failed implants compared to 6% among subjects with teeth missing due to trauma or agenesis.

    The main conclusions of this thesis were that (i) a check of whether one or more permanent incisors are missing will identify 65% of individuals with oligodontia and 84% of individuals missing nine teeth or more, (ii) evaluation of salivary secretion is indicated in children with oligodontia, (iii) a majority of individuals with oligodontia did not report other abnormal ectodermal organ function besides teeth, (iv) no clinical indicator discriminated between individuals with and without mutations in the tested genes, and more unidentified genes are involved in tooth morphogenesis, (v) EDARADD mutations are associated with isolated oligodontia, (vi) evaluation of orofacial function is indicated in individuals with ED, and many individuals with ED would benefit from orofacial skills training, (vii) dental implant placement is a rare treatment modality in children, (viii) individuals with hypohidrotic ED seem to present special challenges due to structural as well as direct effects of the mutations on bone, which seem to compromise osseointegration, (ix) central registers on signs and symptoms in individuals with rare disorders would help establish prevalences of various diagnoses and define treatment needs, and (x) quality registers for monitoring treatment outcomes of dental implants would promote early detection of risks and side-effects in individuals with rare disorders.

  • 28.
    Bergendal, Birgitta
    et al.
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi, Pedodonti.
    Ekman, Agneta
    Nilsson, Peter
    Implant failure in young children with ectodermal dysplasia: a retrospective evaluation of use and outcome of dental implant treatment in children in Sweden2008Inngår i: International Journal of Oral & Maxillofacial Implants, ISSN 0882-2786, E-ISSN 1942-4434, Vol. 23, nr 3, s. 520-524Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Purpose: This study surveyed dental implant treatment in children up to age 16 years in Sweden between 1985 and 2005, with special reference to young children with ectodermal dysplasia (ED) and anodontia in the lower jaw.

    Materials and Methods: A questionnaire was sent to Swedish specialist clinics in oral and maxillofacial surgery and prosthetic dentistry. Also, the teams who had treated children with ED were asked to submit their records for these children for a discussion on reasons for implant failure.

    Results: Six out of 30 specialist centers (20%) in Sweden had treated 26 children with dental implants between 1985 and 2005. Twenty-one patients had received 33 implants to replace teeth missing from nonsyndromic agenesis or trauma at ages 14 or 15 years; 2 (6.1%) of these implants were lost. Five children with ED received 14 implants at 5 to 12 years of age; 9 (64.3%) of these implants were lost before loading.

    Conclusions: Dental implant placement has been a rarely used treatment modality in Swedish children less than 16 years old in the last 20 years. The failure rate in children treated because of tooth agenesis was only slightly higher than that reported for adult individuals, whereas in young children with ED and anodontia in the mandible, implants seemed to present special challenges, and the failure rate was very high. The small jaw size and peroperative conditions, rather than ED per se, were thought to be the main risk factors. Centralizing implant operations in young children with ED and monitoring outcomes in implant registers are strongly advocated. Int J Oral Maxillofacial Implants 2008;23:520–524

  • 29. Bergendal, Birgitta
    et al.
    Klar, Joakim
    Stecksén-Blicks, Christina
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Norderyd, Johanna
    Dahl, Niklas
    Isolated oligodontia associated with mutations in EDARADD, AXIN2, MSX1, and PAX9 genes2011Inngår i: American Journal of Medical Genetics. Part A, ISSN 1552-4825, E-ISSN 1552-4833, Vol. 155, nr 7, s. 1616-1622Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Oligodontia is defined as the congenital lack of six or more permanent teeth, excluding third molars. Oligodontia as well as hypodontia (lack of one or more permanent teeth) are highly heritable conditions associated with mutations in the AXIN2, MSX1, PAX9, EDA, and EDAR genes. Here we define the prevalence of mutations in the AXIN2, MSX1, PAX9, EDA, and EDAR genes, and the novel candidate gene EDARADD in a cohort of 93 Swedish probands with non-syndromic, isolated oligodontia. Mutation screening was performed using denaturing gradient gel electrophoresis and DNA sequence analysis. Analyses of the coding sequences of the six genes showed sequence alterations predicted to be damaging or potentially damaging in ten of 93 probands (10.8%). Mutations were identified in the EDARADD (n = 1), AXIN2 (n = 3), MSX1 (n = 2), and PAX9 (n = 4) genes, respectively. None of the 10 probands with mutations had other self-reported symptoms from ectodermal tissues. The oral parameters were similar when comparing individuals with and without mutations but a family history of oligodontia was three times more frequent for probands with mutations. EDARADD mutations have previously been reported in a few families segregating hypohidrotic ectodermal dysplasia and this is, to our knowledge, the first report of an EDARADD mutation associated with isolated oligodontia.

  • 30.
    Bergendal, Birgitta
    et al.
    Umeå universitet, Medicinsk fakultet, Odontologi. Umeå universitet, Medicinsk fakultet, Odontologi, Pedodonti.
    McAllister, Anita
    Stecksen-Blicks, Christina
    Umeå universitet, Medicinsk fakultet, Odontologi. Umeå universitet, Medicinsk fakultet, Odontologi, Pedodonti.
    Orofacial dysfunction in ectodermal dysplasias measured using the Nordic Orofacial Test-Screening protocol2009Inngår i: Acta Odontologica Scandinavica, ISSN 0001-6357, E-ISSN 1502-3850, ISSN 1502-3850, Vol. 67, s. 377-381Artikkel i tidsskrift (Fagfellevurdert)
  • 31.
    Bergendal, Birgitta
    et al.
    Odontologiska Institutionen, Jönköping.
    Norderyd, J
    Bågesund, M
    Holst, A
    Signs and symptoms from ectodermal organs in young Swedish individuals with oligodontia2006Inngår i: International Journal of Paediatric Dentistry, ISSN 0960-7439, E-ISSN 1365-263X, Vol. 16, nr 5, s. 320-326Artikkel i tidsskrift (Fagfellevurdert)
  • 32.
    Bergendal, Birgitta
    et al.
    Odontologiska Institutionen, Jönköping.
    Stecksén-Blicks, C
    Gabriel, H
    Norderyd, J
    Dahl, N
    Isolated oligodontia associated with mutations in AXIN2, MSX1, PAX9, and EDARADDManuskript (preprint) (Annet vitenskapelig)
  • 33.
    Berglund, Anders
    et al.
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Branting-Ekenbäck, C
    Ohlson, M
    En översikt över chairside CAD/CAM-system i Sverige. Garanteras patientsäkerheten genom CE-märkningen av utrustning och material?2013Rapport (Annet vitenskapelig)
    Abstract [sv]

    Inom odontologin har Computer-Aided Design/Computer-Aided Manufacturing (CAD/CAM) blivit allt vanligare, främst på tandtekniska laboratorier, men tekniken blir också vanligare på tandläkarklinikerna, s.k. chairside CAD/CAM. Chairside CAD/CAM innebär att tandläkaren efter preparationen av en tand framställer tandersättningen i tre steg. I första steget används en intraoral scanner istället för den traditionella avtryckstagningen (Mörmann et al., 2002; Beuer et al., 2008; Hehn, 2001). Man kan även scanna modeller och avtryck, vilket kan vara bra om patienten har svårt att gapa eller har hög salivproduktion, då scannrarna är känsliga för fuktiga miljöer (Kachalia et al., 2010). Det digitala avtrycket överförs till datorn, där uppgifterna bearbetas och en modell av tänderna skapas i 3D. I det andra steget designar tandläkaren tandersättningen på 3D-modellen. Här bestämmer tandläkaren kusphöjd, kontakter approximalt, utsträckning mot preparationsgränsen och utseende på tandersättningen. I det tredje steget fräses tandersättningen fram ur ett keramblock i en fräsmaskin. Ofta målas kronan här för att karaktärisera den och ge den ett mer tandlikt utseende innan den bränns i ugn. Med större möjligheter att själv kunna sköta hela processen fram till färdig tandkonstruktion måste tandläkaren fundera över hur kvaliteten och säkerheten hos de färdiga produkterna kan säkerställas. Färdiga tandtekniska arbeten räknas som specialanpassade medicintekniska produkter och ska inte CE-märkas men de material som ingår i arbetet är oftast CE-märkta. Lag (1993:584) om medicintekniska produkter och Läkemedelsverkets föreskrifter (LVFS 2003:11) om medicintekniska produkter innehåller krav på att medicintekniska produkter som släpps ut på marknaden ska vara lämpliga och säkra för sina användningsområden genom att krav ställs som ska leda till att allvarliga avvikelser, olyckor och tillbud på grund av produkterna så långt som möjligt ska kunna undvikas. Avvikelser inkluderar här både funktionsfel och misstänkta biverkningar. Innan ett tandtekniskt laboratorium får börja leverera tandtekniska arbeten till tandläkare måste ett antal krav vara uppfyllda för att bl.a. säkerställa säkerheten hos dessa produkter för patienterna. Laboratoriet ska vara registrerat hos Läkemedelsverket och vid registreringen måste man intyga att man har en tillverkningsverksamhet som uppfyller de krav för specialanpassade medicintekniska produkter som ställs i LVFS 2003:11. Medicintekniska produkter ska konstrueras och tillverkas på ett sådant sätt att de inte äventyrar patienternas kliniska tillstånd eller säkerhet, användarnas eller i förekommande fall andra personers hälsa och säkerhet, när de används under avsedda förhållanden och för sitt avsedda ändamål. Riskerna med att använda produkterna ska vara acceptabla med tanke på fördelarna för patienten och förenliga med en hög hälso-och säkerhetsnivå. Detta innefattar omfattande krav både på produkt och tillverkningsprocess. Det finns en del oklarheter rörande hur det regelverk som styr framställningen av tandtekniska arbeten tillämpas för arbeten tillverkade med chairside CAD/CAM.

  • 34.
    Berglund, Anders
    et al.
    Umeå universitet, Medicinsk fakultet, Odontologi, Tandteknikerprogrammet.
    Hulterström, Anna Karin
    Umeå universitet, Medicinsk fakultet, Odontologi, Tandteknikerprogrammet.
    Gruffman, Ewa
    Umeå universitet, Medicinsk fakultet, Odontologi, Odontologisk materialvetenskap.
    van Dijken, Jan
    Umeå universitet, Medicinsk fakultet, Odontologi, Tandhygienistprogrammet.
    Dimensional change of a calcium aluminate cement for posterior restorations in aqueous and dry media.2006Inngår i: Dental Materials, ISSN 0109-5641, E-ISSN 1879-0097, Vol. 22, nr 5, s. 470-476Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    OBJECTIVES: A calcium aluminate cement has recently been developed, with claims of being an alternative to dental amalgam and resin composites in posterior cavities. However, its' mechanical properties are not well evaluated and the aim of the study was therefore, to evaluate its' dimensional stability over time. METHODS: The dimensional changes of the cement, Doxadent, and two composite resins, Esthet-X and InTen-S, were tested during 360 d. The specimens were stored at 37+/-1 degrees C either in 100% air humidity (dry) or immersed in distilled water (wet), except for the first 24h when all specimens were stored at 100% air humidity and 37+/-1 degrees C. RESULTS: During the first 24h, Doxadent decreased in volume with 0.04%, while InTen-S and Esthet-X decreased with 1.60 and 1.75%, respectively. From d 1-360, the dry Doxadent specimens increased in volume with 2.0% and in weight with 5.5%, while the corresponding increase for the wet specimens were 4.1 and 6.3%, respectively. The volume of both composites increased 0.8% or less in dry and wet conditions, while the increase in weight for InTen-S was 1.2% for the wet specimens and 0.6% for the dry. The corresponding figures for Esthet-X were 0.7 and 0.2%. SIGNIFICANCE: Doxadent was less dimensionally stable than the composites tested. Doxadent increased 2 times more in volume immersed in water than in 100% air humidity, while the increase in weight was almost similar. The clinical implications of the results found in the present study are uncertain. A material that continues to absorb water during prolonged periods and continues to react is questionable for clinical use.

  • 35.
    Bergman, Bo
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    The distribution and concentration of zinc and the effects of zinc deficiency in the mammalian body: some experiments in mice and rats with special reference to mandibular condyle and some other skeletal tissues1970Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    This thesis constitutes a summary of the following series of studies:

    I Autoradiographic studies on the distribution of zinc-65 in mice. In collaboration with Rune Söremark. The Journal of Nutrition 94,6, 1968.

    II Comparative study of distribution of injected zinc 65 in the mandibularcondyle and other tissues in rat as determined by gammascintillation. Acta Radiologica, Therapy Physics Biology 1970. Preprint.

    III Concentration of zinc in some hard and soft tissues of rat determinedby neutron activation analysis. Acta Radiologica, TherapyPhysics Biology 1970. Preprint.

    IV The distribution of 65Zn in the endochondral growth sites of themandibular condyle and the proximal end of the tibia in youngrats.—An autoradiographic and gamma scintillation study. OdontologiskRevy, 1970. Preprint.

    V Morphologic and autoradiographic observations on the effect ofzinc deficiency on endochondral growth sites in the white rat. Incollaboration with Ulf Friberg, Stefan Lohmander and TorstenÖberg. Odontologisk Revy, 1970. Preprint.

    VI The zinc concentration in hard and soft tissues of the rat. Theinfluence of zinc deficient feeding. Acta odontologica Scandinavica,1970. Preprint.

    These studies will be referred to in the following by the appropriate Roman numerals.

  • 36.
    Bergman, Maud
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
    Chemical and thermodynamic studies of dental gold alloys with special reference to homogenization, electrochemical corrosion and cluster formation1976Doktoravhandling, med artikler (Annet vitenskapelig)
  • 37. Bersani, Cinzia
    et al.
    Mints, Michael
    Umeå universitet, Medicinska fakulteten, Institutionen för kirurgisk och perioperativ vetenskap. Dept. of Medicine, Karolinska Institutet, Stockholm, Sweden.
    Tertipis, Nikolaos
    Haeggblom, Linnea
    Sivars, Lars
    Ährlund-Richter, Andreas
    Vlastos, Andrea
    Smedberg, Cecilia
    Grün, Nathalie
    Munck-Wikland, Eva
    Näsman, Anders
    Ramqvist, Torbjörn
    Dalianis, Tina
    A model using concomitant markers for predicting outcome in human papillomavirus positive oropharyngeal cancer2017Inngår i: Oral Oncology, ISSN 1368-8375, E-ISSN 1879-0593, Vol. 68, s. 53-59Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Objective: Head-neck cancer therapy has become intensified. With radiotherapy alone, 3-year disease-free survival (DFS) is 80% for HPV-positive TSCC/BOTSCC and better for patients with favorable characteristics, suggesting therapy could be tapered for some, decreasing side-effects. Therefore, we built a model to predict progression-free survival for patients with HPV-positive TSCC and BOTSCC. Material and methods: TSCC/BOTSCC patients treated curatively between 2000 and 2011, with HPV16 DNA/E7 mRNA positive tumors examined for CD8(+) TILs, HPV16 mRNA and HLA class I expression were included. Patients were split randomly 65/35 into training and validation sets, and LASSO regression was used to select a model in the training set, the performance of which was evaluated in the validation set. Results: 258 patients with HPV DNA/E7 mRNA positive tumors could be included, 168 and 90 patients in the respective sets. No treatment improved prognosis compared to radiotherapy alone. CD8(+) TIL counts and young age were the strongest predictors of survival, followed by T-stage <3 and presence of HPV16 E2 mRNA. The model had an area under curve (AUC) of 76%. A model where the presence of three of four of these markers defined good prognosis captured 56% of non-relapsing patients with a positive predictive value of 98% in the validation set. Furthermore, the model identified 35% of our cohort that was over-treated and could safely have received de-escalated therapy. Conclusion: CD8(+) TIL counts, age, T-stage and E2 expression could predict progression-free survival, identifying patients eligible for randomized trials with milder treatment, potentially reducing side effects without worsening prognosis.

  • 38. Björnström, H
    et al.
    Naji, S
    Simic, D
    Sjöström, Inger
    Umeå universitet, Medicinsk fakultet, Odontologi, Pedodonti.
    Twetman, Svante
    Umeå universitet, Medicinsk fakultet, Odontologi, Pedodonti.
    Fluoride levels in saliva and dental plaque after consumption of snacks prepared with fluoridated salt.2004Inngår i: European journal of paediatric dentistry, Vol. 5, nr 1, s. 41-5Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [sv]

    AIM: To determine the concentration of fluoride in saliva and supragingival dental plaque at designated time intervals after consumption of snacks prepared with a standardised amount of fluoridated salt. STUDY DESIGN: The investigation had a single blind prospective crossover design. METHODS: A group of 11 healthy young adults volunteered to participate after verbal and written information and consent. After a 1-week fluoride depletion period, the subjects consumed popcorn prepared with either fluoridated (250 mg/kg) or non-fluoridated salt during 30 minutes. Unstimulated whole saliva and samples of supragingival plaque were collected before consumption (baseline) and at 30, 60 and 120 minutes after the intake. Fluoride concentration was determined with a fluoride-specific electrode and the post-ingestion levels were compared with baseline by ANOVA. RESULTS: In saliva, the mean fluoride concentrations at baseline ranged from 0.021 to 0.027 mg/L and after the 30 minutes consumption of fluoride prepared snacks a 15-fold increase (p<0.001) was found. The same pattern was disclosed in the plaque samples. In both saliva and plaque, the fluoride levels remained significantly elevated after 2 hours (p<0.001 and p<0.05, respectively). CONCLUSION: Consumption of snacks prepared with fluoridated table salt resulted in significantly increased fluoride levels in saliva and supragingival plaque for a period of at least two hours.

  • 39.
    Blind, Anna
    et al.
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk vetenskap, Öron- näs- och halssjukdomar.
    Hulterström, Antti
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Berggren, Diana
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk vetenskap, Öron- näs- och halssjukdomar.
    Treatment of nasal septal perforations with a custom-made prosthesis2009Inngår i: European Archives of Oto-Rhino-Laryngology, ISSN 0937-4477, E-ISSN 1434-4726, Vol. 266, nr 1, s. 65-69Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    We present the fabrication and clinical use of a custom-made nasal septal silicone button that can be inserted transnasally into a perforation of the nasal septum by the physician as an office procedure, or by the patients themselves in their home. Questionnaire and retrospective chart review were used to evaluate the efficacy of this prosthesis as treatment of disturbing symptoms from nasal septal perforation. The study included 41 patients (27 women) with a nasal septal perforation. The follow-up time ranged from 1 to 9 years. Symptoms investigated were nasal obstruction, crusting, feeling of dryness, pain, epistaxis, and whistling from the nose. The degree of experienced symptoms was estimated on a VAS-scale. The questionnaire was answered by 37 of the 41 patients. Fourteen patients were still using their button at the follow-up. Treatment with the prosthesis greatly diminished all the investigated symptoms. Also, use of the silicone button resulted in an improved quality of life. No case of infection was noted in connection with use of the silicone prosthesis.

  • 40.
    Blomstedt, Patric
    Umeå universitet, Medicinska fakulteten, Institutionen för farmakologi och klinisk neurovetenskap, Klinisk neurovetenskap.
    Dental surgery in ancient Egypt2013Inngår i: Journal of the history of dentistry, ISSN 1089-6287, Vol. 61, nr 3, s. 129-142Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Many different surgical procedures have over the years been attributed to the ancient Egyptians. This is also true regarding the field of dental surgery. The existence of dentists in ancient Egypt is documented and several recipes exist concerning dental conditions. However, no indications of dental surgery are found in the medical papyri or in the visual arts. Regarding the osteological material/mummies, the possible indications of dental surgery are few and weak. There is not a single example of a clear tooth extraction, nor of a filling or of an artificial tooth. The suggested examples of evacuation of apical abscesses can be more readily explained as outflow sinuses. Regarding the suggested bridges, these are constituted of one find likely dating to the Old Kingdom, and one possibly, but perhaps more likely, dating to the Ptolemaic era. Both seem to be too weak to have served any possible practical purpose in a living patient, and the most likely explanation would be to consider them as a restoration performed during the mummification process. Thus, while a form of dentistry did certainly exist in ancient Egypt, there is today no evidence of dental surgery.

  • 41.
    Boldrup, Linda
    et al.
    Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Patologi.
    Coates, Philip J
    Laurell, Göran
    Umeå universitet, Medicinska fakulteten, Institutionen för klinisk vetenskap, Öron- näs- och halssjukdomar.
    Nylander, Karin
    Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap, Patologi.
    Differences in p63 expression in SCCHN tumours of different sub-sites within the oral cavity2011Inngår i: Oral Oncology, ISSN 1368-8375, E-ISSN 1879-0593, Vol. 47, nr 9, s. 861-865Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Squamous cell carcinoma of the head and neck, SCCHN, the sixth most common cancer in the world, comprises tumours of differentanatomical sites. The overall survival is low, and there are no good prognostic or predictive markers available. The p53 homologue, p63, plays an important role in development of epithelial structures and has also been suggested to be involved in development of SCCHN. However, most studies on p63 in SCCHN have not taken into account the fact that this group of tumours is heterogeneous in terms of the particular site of origin of the cancer. Mapping and comparing p63 expression levels in tumours and corresponding clinically normal tissue in SCCHN from gingiva, tongue and tongue/floor of the mouth revealed clear differences between these regions. In normal samples from tongue and gingiva, tongue samples showed 2.5-fold higher median p63 expression and also more widespread expression compared to gingival samples. These results emphasise the importance of taking sub-site within the oral cavity into consideration in analyses of SCCHN.

  • 42. Bondemark, L
    et al.
    Holm, AK
    Umeå universitet, Medicinsk fakultet, Odontologi, Pedodonti.
    Hansen, K
    Mohlin, S
    Brattström, V
    Paulin, G
    Pietila, T
    Long-term stability of orthodontic treatment and patient satisfaction. A systematic review.2007Inngår i: The Angle orthodontist, Vol. 77, nr 1, s. 181-91Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    OBJECTIVE: To evaluate morphologic stability and patient satisfaction at least 5 years after orthodontic treatment. MATERIALS AND METHODS: Published literature was searched through the PubMed and Cochrane Library electronic databases from 1966 to January 2005. The search was performed by an information specialist at the Swedish Council on Technology Assessment in Health Care. The inclusion criteria consisted of a follow-up period of at least 5 years postretention; randomized clinical trials, prospective or retrospective clinical controlled studies, and cohort studies; and orthodontic treatment including fixed or removable appliances, selective grinding, or extractions. Two reviewers extracted the data independently and also assessed the quality of the studies. RESULTS: The search strategy resulted in 1004 abstracts or full-text articles, of which 38 met the inclusion criteria. Treatment of crowding resulted in successful dental alignment. However, the mandibular arch length and width gradually decreased, and crowding of the lower anterior teeth reoccurred postretention. This condition was unpredictable at the individual level (limited evidence). Treatment of Angle Class II division 1 malocclusion with Herbst appliance normalized the occlusion. Relapse occurred but could not be predicted at the individual level (limited evidence). The scientific evidence was insufficient for conclusions on treatment of cross-bite, Angle Class III, open bite, and various other malocclusions as well as on patient satisfaction in a long-term perspective. CONCLUSIONS: This review has exposed the difficulties in drawing meaningful evidence-based conclusions often because of the inherent problems of retrospective and uncontrolled study design.

  • 43. Boström, Elisabeth A.
    et al.
    Kindstedt, Elin
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Sulniute, Rima
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Palmqvist, Py
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Majster, Mirjam
    Holm, Cecilia Koskinen
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Zwicker, Stephanie
    Clark, Reuben
    Önell, Sebastian
    Johansson, Ingegerd
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Lerner, Ulf H.
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi. Univ Gothenburg, Sahlgrenska Acad, Ctr Bone & Arthrit Res, Gothenburg, Sweden.
    Lundberg, Pernilla
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Increased Eotaxin and MCP-1 Levels in Serum from Individuals with Periodontitis and in Human Gingival Fibroblasts Exposed to Pro-Inflammatory Cytokines2015Inngår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 10, nr 8, artikkel-id e0134608Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Periodontitis is a chronic inflammatory disease of tooth supporting tissues resulting in periodontal tissue destruction, which may ultimately lead to tooth loss. The disease is characterized by continuous leukocyte infiltration, likely mediated by local chemokine production but the pathogenic mechanisms are not fully elucidated. There are no reliable serologic biomarkers for the diagnosis of periodontitis, which is today based solely on the degree of local tissue destruction, and there is no available biological treatment tool. Prompted by the increasing interest in periodontitis and systemic inflammatory mediators we mapped serum cytokine and chemokine levels from periodontitis subjects and healthy controls. We used multivariate partial least squares (PLS) modeling and identified monocyte chemoattractant protein-1 (MCP-1) and eotaxin as clearly associated with periodontitis along with C-reactive protein (CRP), years of smoking and age, whereas the number of remaining teeth was associated with being healthy. Moreover, body mass index correlated significantly with serum MCP-1 and CRP, but not with eotaxin. We detected higher MCP-1 protein levels in inflamed gingival connective tissue compared to healthy but the eotaxin levels were undetectable. Primary human gingival fibroblasts displayed strongly increased expression of MCP-1 and eotaxin mRNA and protein when challenged with tumor necrosis factor-alpha (TNF-alpha and interleukin-1 beta (IL-1 beta), key mediators of periodontal inflammation. We also demonstrated that the upregulated chemokine expression was dependent on the NF-kappa B pathway. In summary, we identify higher levels of CRP, eotaxin and MCP-1 in serum of periodontitis patients. This, together with our finding that both CRP and MCP-1 correlates with BMI points towards an increased systemic inflammatory load in patients with periodontitis and high BMI. Targeting eotaxin and MCP-1 in periodontitis may result in reduced leukocyte infiltration and inflammation in periodontitis and maybe prevent tooth loss.

  • 44. Boström, Elisabeth A.
    et al.
    Lundberg, Pernilla
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    The Newly Discovered Cytokine IL-34 Is Expressed in Gingival Fibroblasts, Shows Enhanced Expression by Pro-Inflammatory Cytokines, and Stimulates Osteoclast Differentiation2013Inngår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, nr 12, s. e81665-Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Background: Interleukin-34 (IL-34) is a recently discovered cytokine functionally overlapping macrophage colony stimulating factor (M-CSF), a mediator of inflammation and osteoclastogenesis in bone-degenerative diseases such as rheumatoid arthritis. The objective of this study was to assess the expression of IL-34 in human gingival fibroblasts and investigate if the pro-inflammatory cytokines tumor necrosis factor alpha (TNF-alpha) and Interleukin-1B (IL-1 beta) modulate its expression, and moreover if IL-34 could contribute to recruitment of bone-resorbing osteoclasts. Methods: IL-34 expression was evaluated in gingival fibroblasts by real time PCR following stimulation by TNF-alpha, IL-1 beta, and treatment with inhibitors of intracellular pathways. The formation of osteoclasts was evaluated by tartrate-resistant acid phosphatase (TRAP) staining of bone marrow macrophages treated with IL-34 or M-CSF in addition to receptor activator of nuclear factor kappa-B ligand (RANKL). Results: IL-34 was expressed in gingival fibroblasts. The expression was enhanced by TNF-alpha and IL-1 beta, regulated by the transcription factor nuclear factor kappa B (NF-kappa B) and activation of c-Jun N-terminal kinase (JNK). Further, IL-34 supports RANKL-induced osteoclastogensis of bone marrow macrophages, independently of M-CSF. Summary: In conclusion, this study shows for the first time IL-34 expression in human gingival fibroblasts, stimulated by TNF-alpha and IL-1 beta, key mediators of periodontal inflammation. Furthermore, IL-34 can be substituted for M-CSF in RANKL-induced osteoclastogenesis. IL-34 may contribute to inflammation and osteoclastogenesis in bone-degenerative diseases such as periodontitis.

  • 45. Bou Serhal, C
    et al.
    Jacobs, R
    Flygare, Lennart
    Quirynen, M
    van Steenberghe, D
    Perioperative validation of localisation of the mental foramen2002Inngår i: Dento-Maxillo-Facial Radiology, ISSN 0250-832X, E-ISSN 1476-542X, Vol. 31, nr 1, s. 39-43Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    AIM: To assess the accuracy of panoramic radiography and spiral or computed tomography for the localisation of the mental foramen.

    MATERIALS AND METHODS: The distance from the alveolar crest to the mental foramen was measured from panoramic radiographs, spiral tomograms and CT scans. The same distance was measured during implant surgery using a specially designed caliper.

    RESULTS: Panoramic radiography showed more deviation (+0.6 mm) from the perioperative measurements than either spiral or computed tomography (+0.4 and -0.3 mm respectively). The difference was significant (P<0.05). In general, distances were overestimated on the panoramic radiographs.

    CONCLUSIONS: Cross-sectional imaging techniques are recommended for the pre-operative planning of implants in the posterior mandible.

  • 46. Bougas, Kostas
    et al.
    Ransjö, Maria
    Johansson, Anders
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi, Molekylär paradontologi.
    Effects of Porphyromonas gingivalis surface-associated material on osteoclast formation2013Inngår i: Odontology: official journal of The Society of the Nippon Dental University, ISSN 1618-1247, E-ISSN 1618-1255, Vol. 101, nr 2, s. 141-149Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Porphyromonas gingivalis strongly correlates with periodontitis, but the underlying mechanisms causing dentoalveolar bone resorption are not fully understood. As contradictory effects of P. gingivalis on osteoclastogenesis have been reported, this study investigates the effect of P. gingivalis extract on osteoclast formation. Osteoclast formation in mouse bone marrow (MBM) cell cultures and RAW 264.7 cells was stimulated by nuclear factor-κB ligand (RANKL) or parathyroid hormone (PTH). Cells were cultured with and without P. gingivalis surface-associated material and phenotypic characteristics were examined using microscopy, flow cytometry, and RT-PCR. P. gingivalis significantly decreased osteoclast formation and the expression of osteoclast phenotypic markers in PTH-stimulated MBM cultures. Additionally, P. gingivalis inhibited expression of osteoclast differentiation factors and stimulated expression of the mouse macrophage marker F4/80. The presence of P. gingivalis in RANKL-stimulated MBM cultures and RAW 264.7 cells inhibited osteoclastogenesis. Interestingly, a transient exposure with P. gingivalis before PTH stimulation increased osteoclastogenesis in MBM cultures. Flow cytometric analyses of cells transiently exposed to P. gingivalis demonstrated an increased proportion of potential osteoclast precursor cells. We conclude that a transient exposure of MBM cultures to P. gingivalis increases the number of osteoclast precursors and osteoclast formation, whereas a prolonged exposure completely abolishes osteoclastogenesis.

  • 47.
    Brady, L. Jeannine
    et al.
    Department of Oral Biology, University of Florida, Gainesville, FL 32610, USA.
    Maddocks, Sarah E.
    School of Oral and Dental Sciences, University of Bristol, Bristol BS1 2LY, UK.
    Larson, Matthew R.
    Department of Physiology and Biophysics, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
    Forsgren, Nina
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi, Kariologi.
    Persson, Karina
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Deivanayagam, Champion C.
    Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
    Jenkinson, Howard F.
    School of Oral and Dental Sciences, University of Bristol, Bristol BS1 2LY, UK.
    The changing faces of Streptococcus antigen I/II polypeptide family adhesins2010Inngår i: Molecular Microbiology, ISSN 0950-382X, E-ISSN 1365-2958, Vol. 77, nr 2, s. 276-286Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Streptococcus mutans antigen I/II (AgI/II) protein was one of the first cell wall-anchored adhesins identified in Gram-positive bacteria. It mediates attachment of S. mutans to tooth surfaces and has been a focus for immunization studies against dental caries. The AgI/II family polypeptides recognize salivary glycoproteins, and are also involved in biofilm formation, platelet aggregation, tissue invasion and immune modulation. The genes encoding AgI/II family polypeptides are found among Streptococcus species indigenous to the human mouth, as well as in Streptococcus pyogenes, S. agalactiae and S. suis. Evidence of functionalities for different regions of the AgI/II proteins has emerged. A sequence motif within the C-terminal portion of Streptococcus gordonii SspB (AgI/II) is bound by Porphyromonas gingivalis, thus promoting oral colonization by this anaerobic pathogen. The significance of other epitopes is now clearer following resolution of regional crystal structures. A new picture emerges of the central V (variable) region, predicted to contain a carbohydrate-binding trench, being projected from the cell surface by a stalk formed by an unusual association between an N-terminal α-helix and a C-terminal polyproline helix. This presentation mode might be important in determining functional conformations of other Gram-positive surface proteins that have adhesin domains flanked by α-helical and proline-rich regions.

    Ever since dental caries (tooth decay) was first shown to be caused by bacteria, there has been continued interest in developing vaccine or passive immunization protocols for its control or prevention (Lehner et al., 1980). Although dental caries is not fatal, and in developed countries caries is now considered to be largely avoidable through controlled diet and good oral hygiene, there remain significant problems with childhood disease, especially among indigent populations. Consequently, caries is one of the most common worldwide infectious diseases. Therefore, research continues towards employing vaccine formulations comprised of peptide components derived from surface proteins of Streptococcus mutans, a major agent associated with dental caries (Lehner et al., 1975). One of the most promising strategies seems to be delivery of peptides, derived from glucan-binding protein B (GbpB) and antigen I/II (AgI/II) protein, via a mucosal (nasal) route. The GbpB polypeptide binds extracellular glucans, thus promoting co-adhesion of S. mutans cells in the development of dental plaque (Taubman and Nash, 2006). The AgI/II protein (also named P1, SpaP, AgB or PAc) is a major surface protein that functions as an adhesin, attaching S. mutans to the saliva-coated tooth enamel surface (Koga et al., 1990; Kelly et al., 1995). Antibodies against SpaP and GbpB block adherence and co-adhesion, respectively, thus disrupting colonization of the oral cavity by S. mutans (Ma et al., 1990; 1998; Taubman and Nash, 2006).

    The terminology AgI/II derives from the identification of two major cell wall antigens I and II in S. mutans by Russell et al. (1980), and the subsequent recognition that AgII was a component of AgI. Following the discovery of AgI/II, it became apparent that genes encoding orthologous proteins were widely dispersed among the streptococci (Jenkinson and Demuth, 1997). The viridans Streptococcus AgI/II adhesins range in composition from 1310 to 1653 amino acid (aa) residues, while the Streptococcus agalactiae AgI/II proteins are smaller (826–932 aa residues) (Tettelin et al., 2005). The widespread distribution of these AgI/II protein genes across the streptococci is perhaps not surprising, given the complex streptococcal communities that exist on surfaces of the oro- and naso-pharynx and within the bacterial soup of saliva. It is interesting, though, that the AgI/II family polypeptide genes have not yet been discovered in Streptococcus pneumoniae, which might be by the fact that S. pneumoniae forms a distinct evolutionary cluster (Kilian et al., 2008).

  • 48. Brown, J
    et al.
    Jacobs, R
    Levring Jäghagen, Eva
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi, Tandläkarutbildning.
    Lindh, C
    Baksi, G
    Schulze, D
    Schulze, R
    Basic training requirements for the use of dental CBCT by dentists: a position paper prepared by the European Academy of DentoMaxilloFacial Radiology2014Inngår i: Dento-Maxillo-Facial Radiology, ISSN 0250-832X, E-ISSN 1476-542X, Vol. 43, nr 1, artikkel-id 20130291Artikkel i tidsskrift (Fagfellevurdert)
    Abstract [en]

    Cone beam CT (CBCT) is a relatively new imaging modality, which is now widely available to dentists for examining hard tissues in the dental and maxillofacial regions. CBCT gives a three-dimensional depiction of anatomy and pathology, which is similar to medical CT and uses doses generally higher than those used in conventional dental imaging. The European Academy of DentoMaxilloFacial Radiology recognizes that dentists receive training in two-dimensional dental imaging as undergraduates, but most of them have received little or no training in the application and interpretation of cross-sectional three-dimensional imaging. This document identifies the roles of dentists involved in the use of CBCT, examines the training requirements for the justification, acquisition and interpretation of CBCT imaging and makes recommendations for further training of dentists in Europe who intend to be involved in any aspect of CBCT imaging. Two levels of training are recognized. Level 1 is intended to train dentists who prescribe CBCT imaging, such that they may request appropriately and understand the resultant reported images. Level 2 is intended to train to a more advanced level and covers the understanding and skills needed to justify, carry out and interpret a CBCT examination. These recommendations are not intended to create specialists in CBCT imaging but to offer guidance on the training of all dentists to enable the safe use of CBCT in the dentoalveolar region.

  • 49.
    Brundin, Malin
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Stability of bacterial DNA in relation to microbial detection in teeth2013Doktoravhandling, med artikler (Annet vitenskapelig)
    Abstract [en]

    The fate of DNA from dead cells is an important issue when interpreting results from root canal infections analysed by the PCR technique. DNA from dead bacterial cells is known to be detectable long time after cell death and its stability is dependent on many different factors. This work investigated factors found in the root canal that could affect the recovery of microbial DNA. In an ex vivo experiment, DNA from non-viable gram-positive Enterococcus faecalis was inoculated in instrumented root canals and recovery of DNA was assessed by PCR over a two-year period. DNA was still recoverable two years after cell death in 21/25 teeth. The fate of DNA from the gram-negative bacteria Fusobacterium nucleatum and the gram-positive Peptostreptococcus anaerobius was assessed in vitro. DNA from dead F. nucleatum and P. anaerobius could be detected by PCR six months post cell death even though it was clear that the DNA was released from the cells due to lost of cell wall integrity during the experimental period. The decomposition rate of extracellular DNA was compared to cell-bound and it was evident that DNA still located inside the bacterium was much less prone to decay than extracellular DNA.

    Free (extracellular) DNA is very prone to decay in a naked form. Binding to minerals is known to protect DNA from degradation. The fate of extracellular DNA was assessed after binding to ceramic hydroxyapatite and dentine. The data showed that free DNA, bound to these materials, was protected from spontaneous decay and from enzymatic decomposition by nucleases.

    The main conclusions from this thesis were: i) DNA from dead bacteria can be detected by PCR years after cell death ex vivo and in vitro. ii) Cell-bound DNA is less prone to decomposition than extracellular DNA. iii) DNA is released from the bacterium some time after cell death. iv) Extracellular DNA bound to hydroxyapatite or dentine is protected from spontaneous decomposition and enzymatic degradation.

  • 50.
    Brundin, Malin
    et al.
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Figdor, David
    Johansson, Anders
    Sjögren, Ulf
    Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
    Preservation of bacterial DNA by binding to dentinManuskript (preprint) (Annet vitenskapelig)
1234567 1 - 50 of 547
RefereraExporteraLink til resultatlisten
Permanent link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf