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  • 1. Bellini, C.
    et al.
    Chupeau, M. C.
    Guerche, P.
    Vastra, G.
    Chupeau, Y.
    Transformation of Lycopersicon peruvianum and Lycopersicon esculentum mesophyll protoplasts by electroporation1989In: Plant Science, ISSN 0168-9452, E-ISSN 1873-2259, Vol. 65, no 1, p. 63-75Article in journal (Refereed)
  • 2. Bellini, C.
    et al.
    Giordani, C.
    Lupotto, E.
    Locatelli, F.
    Cuzzoni, E.
    Avogadro, E.
    Castiglione, S.
    Sala, F.
    Stability of a foreign gene in transgenic Nicotiana tabacum  plants during a cycle of dedifferentiation-differentiation1992In: Plant Science, ISSN 0168-9452, E-ISSN 1873-2259, Vol. 82, no 2, p. 193-200Article in journal (Refereed)
    Abstract [en]

    Protoplasts of Nicotiana tabacum were transformed with the APH(3’)II gene, which confers kanamycin resistance. Plants resistant to kanamycin were differentiated and 3 of them were chosen at random. These were used to study the stability of the foreign gene after a cycle of dedifferentiation, to produce calli, and differentiation, to produce new plants. The effect of the selective pressure was analyzed by performing dedifferentiation and differentiation in the presence or absence of kanamycin. Inbred plants were also produced from the original transformed plants and used as control. Southern blot analysis of DNA extracted from 66 regenerated plants showed in all cases that no detectable alteration occurred both in gene structure and insertion site. Furthermore the specific activity of the APH(3’)II enzyme was shown to be at high level in all regenerated plants regardless of the fact that they were regenerated in the presence or absence of kanamycin. The results described here are experimental evidence that a hybrid forcing gene is rather stable in a heterologous genome even after dedifferentiation of the transformed plants and differentiation in vitro, i.e. in those conditions known to be correlated with extensive somaclonal variation.

  • 3. Carol, R. J.
    et al.
    Breiman, A.
    Erel, N.
    Vittorioso, P.
    Bellini, C.
    PASTICCINO1 (AtFKBP70) is a nuclear-localised immunophilin required during Arabidopsis thaliana embryogenesis2001In: Plant Science, ISSN 0168-9452, E-ISSN 1873-2259, Vol. 161, no 3, p. 527-535Article in journal (Refereed)
    Abstract [en]

    The PASTICCINO1 (PAS1) gene of Arabidopsis thaliana encodes a protein with homology to the FK506-binding protein (FKBP) class of immunophilins. To begin to understand more about the possible function of PAS1, we tested some properties of recombinant PAS1 protein and analysed the expression of the gene in Arabidopsis embryos and cell cultures and in tobacco cells. In pas1-1/+ heterozygote embryos the pas1-1 allele is expressed at very low levels in all cells, but it is misexpressed in the pas1-1 homozygote mutant at the same stage. Anti-PAS1 affinity-purified antibodies recognise a 70 kDa protein from dividing cell cultures of Arabidopsis. In indirect immunofluorescence, the same antibodies label the nuclei of dividing tobacco BY-2 cells. In a protease-coupled assay, recombinant PAS1 protein has low peptidylprolyl cis-trans isomerase (PPIase) activity, which is inhibited by the immunosuppressive drugs FK506 and rapamycin, but not by cyclosporin. PAS1 also binds calmodulin in vitro. This data suggests the importance of the correctly regulated production of functional PAS1 protein, a likely nuclear-localised FKBP, for the correct development of the plant embryo. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

  • 4. NAKAMOTO, H
    et al.
    SUNDBLAD, LG
    Gardeström, Per
    Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
    SUNDBOM, E
    FAR-RED STIMULATED LONG-LIVED LUMINESCENCE FROM BARLEY PROTOPLASTS1988In: Plant Science, ISSN 0168-9452, E-ISSN 1873-2259, Vol. 55, no 1, p. 1-7Article in journal (Refereed)
  • 5. PETIT, PX
    et al.
    Gardeström, Per
    Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
    RASMUSSON, AG
    MOLLER, IM
    PROPERTIES OF SUBMITOCHONDRIAL PARTICLES FROM PLANT-MITOCHONDRIA - GENERATION, SURFACE CHARACTERISTICS AND NAD(P)H OXIDATION1991In: Plant Science, ISSN 0168-9452, E-ISSN 1873-2259, Vol. 78, no 2, p. 177-183Article in journal (Refereed)
    Abstract [en]

    Purified mitochondria isolated from potato (Solunum tuberosum L. cv. Bintje) tuber, Jerusalem artichoke (Helianthus tuberosus L.) tuber and rat liver were disrupted at different pH and different EDTA and MgCl2 concentrations either by French Press treatment or by sonication. The submitochondrial particles (SMP) were isolated by differential centrifugation and polarity estimated by the latency of cytochrome c oxidase (CCO) activity. The SMP were 5-95% inside-out depending on the conditions, and the disruption method was more important than the composition of the disruption medium in determining the polarity. At pH 6 and 7 and high-salt conditions sonication yielded SMP of the same polarity (82-91% inside-out) whereas French Press treatment in a low-salt buffer + EDTA gave more inside-out SMP at pH 6 than at pH 7. The inside-out vesicles were able to build up a membrane potential in the presence of respiratory substrates (as tested with the anionic dye, oxonol VI) whereas no membrane potantial could be detected with the right-side-out vesicles (as tested with cationic dyes, and optical dye, safranine O, and a fluorescent dye, rhodamine 123) under similar conditions. Binding of Concanavalin A indicated that both the inner and outer surface of the inner membrane have exposed glycoproteins and/or glycolipids. Both right-side-out and inside-out SMP oxidized NADH, NADPH and succinate with good rates but there were clear differences in both donor and acceptor specificity between the outer and inner surface of the inner mitochondrial membrane. NADH oxidation by inside-out SMP was Ca2+-independent and rotenone-inhibited whereas NADPH oxidation by the inside-out SMP was Ca2+-dependent and relatively unaffected by rotenone.

  • 6.
    Selstam, Eva
    et al.
    Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
    Oquist, Gunnar
    Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
    EFFECTS OF FROST HARDENING ON THE COMPOSITION OF GALACTOLIPIDS AND PHOSPHOLIPIDS OCCURRING DURING ISOLATION OF CHLOROPLAST THYLAKOIDS FROM NEEDLES OF SCOTS PINE1985In: Plant Science, ISSN 0168-9452, E-ISSN 1873-2259, Vol. 42, no 1, p. 41-48Article in journal (Refereed)
  • 7.
    Tan, Biyue
    et al.
    Umeå University, Faculty of Science and Technology, Department of Ecology and Environmental Sciences. Biomaterials Division, Stora Enso AB, SE-131 04, Nacka, Sweden.
    Grattapaglia, Dario
    Wu, Harry X.
    Ingvarsson, Pär K.
    Genomic relationships reveal significant dominance effects for growth in hybrid Eucalyptus2018In: Plant Science, ISSN 0168-9452, E-ISSN 1873-2259, Vol. 267, p. 84-93Article in journal (Refereed)
    Abstract [en]

    Non-additive genetic effects can be effectively exploited in control-pollinated families with the availability of genome-wide markers. We used 41,304 SNP markers and compared pedigree vs. marker-based genetic models by analysing height, diameter, basic density and pulp yield for Eucalyptus urophylla x E.grandis control-pollinated families represented by 949 informative individuals. We evaluated models accounting for additive, dominance, and first-order epistatic interactions (additive by additive, dominance by dominance, and additive by dominance). We showed that the models can capture a large proportion of the genetic variance from dominance and epistasis for growth traits as those components are typically not independent. We also showed that we could partition genetic variances more precisely when using relationship matrices derived from markers compared to using only pedigree information. In addition, phenotypic prediction accuracies were only slightly increased by including dominance effects for growth traits since estimates of non-additive variances yielded rather high standard errors. This novel result improves our current understanding of the architecture of quantitative traits and recommends accounting for dominance variance when developing genomic selection strategies in hybrid Eucalyptus.

  • 8. WINGSLE, G
    et al.
    MATTSON, A
    EKBLAD, A
    HALLGREN, JE
    Selstam, Eva
    Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
    ACTIVITIES OF GLUTATHIONE-REDUCTASE AND SUPEROXIDE-DISMUTASE IN RELATION TO CHANGES OF LIPIDS AND PIGMENTS DUE TO OZONE IN SEEDLINGS OF PINUS-SYLVESTRIS (L)1992In: Plant Science, ISSN 0168-9452, E-ISSN 1873-2259, Vol. 82, no 2, p. 167-178Article in journal (Refereed)
    Abstract [en]

    Scots pine seedlings (Pinus sylvestris L.) were exposed to ozone at a high-level, 300 ppb 8 h/day for 5 days, and at a low-level, 70-80 ppb 8 h/day for 10 days. In the high-level and low-level experiment the chlorophyll a content decreased 11% and 8%, respectively. The high-level ozone experiment affected the xanthophyll cycle by a 19% higher level of violaxanthin in the exposed seedlings compared to controls at day 5. This was also accompanied by a 42% lower concentration of zeaxanthin. No significant changes of the carotenoids neoxanthin, lutein, alpha-carotene or beta-carotene were found. The molar ratio monogalactosyl diacylglycerol/digalactosyl diacylglycerol decreased by 11% in the high-level ozone exposure. This suggests that the galactolipid:galactolipid galactosyl transferase, located in the outer envelope membrane of the chloroplast, was stimulated by ozone. No significant changes in the total membrane lipid or the acyl group composition was detected. In the high-level ozone experiment the concentration of soluble protein increased in the exposed seedlings during the last 3 days of the experimental period without any change in total protein. No visible damages were observed. The results showed that the activity of the defence enzymes superoxide dismutase (EC 1.15.1.1) and glutathione reductase (EC 1.6.4.2) was not altered after ozone exposure.

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